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Cholesterol and desmosterol incorporation into ram sperm membrane before cryopreservation: Effects on membrane biophysical properties and sperm quality
Carro, María De Las Mercede; Peñalva, Daniel; Antollini, Silvia; Hozbor, Federico Andres; Buschiazzo, Jorgelina (Elsevier, 2020-09)Ram sperm are particularly sensitive to freeze-thawing mainly due to their lipid composition, limiting their use in artificial insemination programs. We evaluated the extent of cholesterol and desmosterol incorporation ... -
Linoleic acid stimulates neutral lipid accumulation in lipid droplets of maturing bovine oocytes
Carro, María De Las Mercedes; Buschiazzo, Jorgelina; Rios, Glenda Laura; Oresti, Gerardo Martin; Alberio, Ricardo (Elsevier, 2013-03-01)Linoleic acid (LA) is a polyunsaturated fatty acid present in high concentrations in bovine follicular fluid; when added to maturation culture media, it affects oocyte competence (depending on the type and concentration ... -
PUFA-rich phospholipid classes and subclasses of ram spermatozoa are unevenly affected by cryopreservation with a soybean lecithin-based extender
Carro, María De Las Mercedes; Luquez, J.M.; Peñalva, D.A.; Buschiazzo, Jorgelina; Hozbor, Federico Andres; Furland, N.E. (Elsevier, 2022-04)Cryopreservation is known to affect spermatozoa structure and function. Ram sperm are among the most highly sensitive mammalian gametes to freezing, due to their lipid composition, which limit their efficiency in artificial ...