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A thermostable GH8 endoglucanase of Enterobacter sp. R1 is suitable for β- glucan deconstruction
Ontañon, Ornella Mailen; Ghio, Silvina; Marrero Diaz De Villegas, Rubén; Garrido, Mercedes Maria; Talia, Paola Mónica; Fehér, Csaba; Campos, Eleonora (Elsevier, 2019-11)Glycoside hydrolase family 8 (GH8) includes endoglucanases, lichenases, chitosanases and xylanases, which are essential for polysaccharides breakdown. In this work, we studied a thermally stable GH8 from the cellulose ... -
Application of In-house xylanases as an addition to a commercial cellulase cocktail for the sustainable saccharification of pretreated blue agave bagasse used for bioethanol production
Montiel, Carmina; Hernández-Meléndez, Oscar; Marques, Susana; Gírio, Francisco; Tavares, João; Ontañon, Ornella Mailen; Campos, Eleonora; Bárzana, Eduardo (MDPI, 2024-08)The study involves the use of commercial cellulase Cellic CTec2 in combination with two in-house xylanases, PxXyn10A (XynA), a recombinant purified enzyme from Paenibacillus xylanivorans A59, and a xylanase enzymatic extract ... -
Evaluation of hydrothermal and alkaline pretreatment routes for xylooligosaccharides production from sugar cane bagasse using different combinations of recombinant enzymes
Capetti, Caio; Arnoldi Pellegrini, Vanessa; Vacilotto, Milena Moreira; da Silva Curvelo, Antonio Aprigio; Falvo, Maurício; Guimaraes, Francisco E.G.; Ontañon, Ornella Mailen; Campos, Eleonora; Polikarpov, Igor (Springer, 2023-10)Xylan is the most abundant constituent of hemicellulose fraction of lignocellulosic biomass. Short xylooligosaccharides (XOS), obtained via xylan hydrolysis, have well-known prebiotic and antioxidant properties that are ... -
Optimisation of xylanases production by two Cellulomonas strains and their use for biomass deconstruction
Ontañon, Ornella Mailen; Bedő, Soma; Ghio, Silvina; Garrido, Mercedes Maria; Topalian, Juliana; Jahola, Dóra; Fehér, Anikó; Valacco, Maria Pia; Campos, Eleonora; Fehér, Csaba (Springer, 2021-05)One of the main distinguishing features of bacteria belonging to the Cellulomonas genus is their ability to secrete multiple polysaccharide degrading enzymes. However, their application in biomass deconstruction still ... -
Production of a bacterial secretome highly efficient for the deconstruction of xylans
Topalian, Juliana; Navas, Laura Emilce; Ontañon, Ornella Mailen; Valacco, Maria Pia; Noseda, Diego Gabriel; Blasco, Martín; Peña, María Jesús; Urbanowicz, Breeanna Rae; Campos, Eleonora (Springer, 2024-09)Bacteria within the Paenibacillus genus are known to secrete a diverse array of enzymes capable of breaking down plant cell wall polysaccharides. We studied the extracellular xylanolytic activity of Paenibacillus xylanivorans ... -
Synergic activity of Cel8Pa β-1,4 endoglucanase and Bg1Pa β-glucosidase from Paenibacillus xylanivorans A59 in beta-glucan conversio
Ghio, Silvina; Bradanini, María B.; Garrido, Mercedes Maria; Ontañon, Ornella Mailen; Piccinni, Florencia Elizabeth; Marrero Diaz De Vill, Rubén; Talia, Paola Mónica; Campos, Eleonora (Elsevier, 2020-12)In the efficient bioconversion of polysaccharides from lignocellulosic biomass, endoglucanases and β-glucosidases are key enzymes for the deconstruction of β-glucans. In this work, we focused on a GH8 endoglucanase ... -
Xylanase production by cellulomonas phragmiteti using lignocellulosic waste materials
Buda, Kata; Fekete, Tünde; Ontañon, Ornella Mailen; Campos, Eleonora; Fehér, Csaba (MDPI, 2024-02)Lignocellulosic biomass holds promise as a renewable feedstock for various applications, but its efficient conversion requires cost-effective degradation strategies. The main objective of this study was to investigate the ...