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Browsing by Author "27318968093"

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    • A synthesis of functional contributions of rhizobacteria to growth promotion in diverse crops 

      Brambilla, Silvina Maricel; Stritzler, Margarita; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (Elsevier, 2022-12)
      The endophytic microbes are known to live asymptomatically inside their host and play crucial roles in the growth, development, fitness, and diversification of plants. The plant-endophyte association ranges from mutualism ...

    • Biotecnología aplicada al mejoramiento genético de alfalfa 

      Grandon, Nancy Gabriela; Moreno, Maria Valeria; Soto, Gabriela Cynthia; Sipowicz, Pablo; Stritzler, Margarita; Pascuan, Cecilia Gabriela; Mamani, Eva Maria Celia (Ediciones INTA, 2022)
      Las diferentes herramientas biotecnológicas desarrolladas durante la última década complementan el mejoramiento convencional, reduciendo el tiempo de obtención de nuevos cultivares y aumentando la ganancia genética en ...

    • CRISPR/Cas9-mediated knockout of a polyester synthase-like gene delays flowering time in alfalfa 

      Galindo Sotomonte, Luisa Fernanda; Jozefkowicz, Cintia; Gomez, Maria Cristina; Stritzler, Margarita; Frare, Romina Alejandra; Bottero, Ana Emilia; Tajima, Hiromi; Blumwald, Eduardo; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Springer, 2023-02)
      Alfalfa (Medicago sativa), commonly known as the “Queen of Forages”, is the main source of plant protein for meat and milk production systems worldwide. Since alfalfa is a perennial vegetative-harvest forage, delaying ...

    • CRISPR/Cas9‑mediated knockout of SPL13 radically increases lettuce yield 

      Beracochea, Valeria Cecilia; Stritzler, Margarita; Radonic, Laura Mabel; Bottero, Ana Emilia; Jozefkowicz, Cintia; Darqui, Flavia Soledad; Ayub, Nicolás Daniel; Lopez Bilbao, Marisa Gisela; Soto, Gabriela Cynthia (Springer, 2022-12)
      Cultivated lettuce (Lactuca sativa L.) is a diploid (2n = 2x = 18), autogamous species. This popular leafy vegetable is considered one of the first crops (Guo et al. 2022). Currently, lettuce is the third most consumed ...

    • Edición génica del inoculante comercial de Alfalfa mediante base-editing por CRISPR-Cas9 

      Serantes, María Laura; Stritzler, Margarita; Brambilla, Silvina Maricel; Ayub, Nicolás Daniel (Ministerio de Desarrollo Agrario - Instituto Tecnológico de Chascomús, 2025-05)
      En países agroexportadores como Argentina, impulsar cultivos sostenibles con menos agroquímicos es clave para reducir costos ambientales y económicos. En este contexto, los inoculantes fijadores de nitrógeno son esenciales ...

    • Edición génica en inoculantes de soja por CRISPR-Cas9 : Mejoramientos para una mayor sostenibilidad agrícola 

      Serantes, María Laura; Stritzler, Margarita; Brambilla, Silvina Maricel; Ayub, Nicolás Daniel (INTA, 2024-11)
      La promoción de cultivos sostenibles con menor uso de agroquímicos es esencial en la agricultura moderna. Los inoculantes fijadores de nitrógeno, como Bradyrhizobium japonicum E109 y SEMIA 5079, utilizados en soja, reducen ...

    • Efficient CRISPR/Cas9 genome editing in alfalfa using a public germplasm 

      Bottero, Ana Emilia; Massa, Gabriela Alejandra; Gonzalez, Matías Nicolás; Stritzler, Margarita; Tajima, Hiromi; Gomez, Maria Cristina; Frare, Romina Alejandra; Feingold, Sergio Enrique; Blumwald, Eduardo; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Frontiers Media, 2021-07)
      Because its ability to acquire large amounts of nitrogen by symbiosis, tetraploid alfalfa is the main source of vegetable proteins in meat and milk production systems in temperate regions. Alfalfa cultivation also adds ...

    • Exploring the cooperation between nitrogen-fixing and non-fixing alfalfa rhizobia 

      Brambilla, Silvina Maricel; Liebrenz, Karen Ivana; Odorizzi, Ariel; Arolfo, Valeria; Maguire, Vanina Giselle; Moreno, Maria Valeria; Ruiz, Oscar Adolfo; Stritzler, Margarita; Serantes, Maria Laura; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (Springer, 2024-07-05)
      Alfalfa plays a crucial role in agriculture due to its nitrogen-fixing partnership with rhizobia, resulting in nutritious forage. While the potential parasitic action of inefficient rhizobia has been extensively studied, ...

    • Generation of a multi‑herbicide‑tolerant alfalfa by using base editing 

      Bottero, Ana Emilia; Gomez, Maria Cristina; Stritzler, Margarita; Tajima, Hiromi; Frare, Romina Alejandra; Pascuan, Cecilia Gabriela; Blumwald, Eduardo; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Springer, 2022-02)
      We present the first report on base editing in alfalfa. Specifically, we showed edited alfalfa with tolerance to both sulfonylurea- and imidazolinone-type herbicides. Alfalfa (Medicago sativa) is one of the most important ...

    • Genome editing of soybean inoculant using CRISPR/Cas9 system: enhancing agricultural sustainability 

      Serantes, Maria Laura; Stritzler, Margarita; Brambilla, Silvina Maricel; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (Springer, 2024-04)
      In modern agriculture, inoculants, consisting of various bacteria and fungi, are essential for promoting crop growth and sustainability while reducing reliance on agrochemicals. Despite their benefits, there have been no ...

    • Identificación del gen DROZIP4 y generación de herramientas moleculares para su eliminación en plantas de soja y alfalfa 

      Blanzaco, Tomás; Soto, Gabriela Cynthia; Stritzler, Margarita (Ministerio de Desarrollo Agrario - Instituto Tecnológico de Chascomús, 2025-05)
      La sequía es uno de los principales factores ambientales que comprometen la supervivencia de los cultivos, afectando a especies de gran importancia para la Argentina, como la alfalfa (Medicago sativa L.) y la soja (Glycine ...

    • Microevolution rather than large genome divergence determines the effectiveness of legume–rhizobia symbiotic interaction under field conditions 

      Jozefkowicz, Cintia; Brambilla, Silvina Maricel; Frare, Romina Alejandra; Stritzler, Margarita; Puente, Mariana Laura; Piccinetti, Carlos Fabian; Soto, Gabriela Cinthia; Ayub, Nicolás Daniel (2017-08)
      Despite the vast screening for natural nitrogenfixing isolates by public and private consortia, no significant progresses in the production of improved nitrogenfixing inoculants for alfalfa production have been made in the ...

    • Obtención de un autopoliploide de Cannabis sativa mediante biotécnicas 

      Iannicelli, Jesica; Patanian, Diego; Rodríguez, Rocio; Bugallo, Veronica Lucia; Coviella, Maria Andrea; Garavaglia, Matías; Stritzler, Margarita; Soto, Gabriela; Maffia, Paulo (REDBIO, 2025-06-24)
      A medida que aumenta la demanda de aceite y/o productos derivados del cannabis, existe una necesidad acuciante de desarrollar mejoras para incrementar la producción de sus metabolitos. Esto puede lograrse por técnicas ...

    • Oligopeptide uptake improves oxidative stress resistance and nodulation competitiveness in soybean rhizobia 

      Brambilla, Silvina Maricel; Liebrenz, Karen Ivana; Frare, Romina Alejandra; Gomez, Maria Cristina; Stritzler, Margarita; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (Elsevier, 2023-12)
      We here explored the mechanisms associated with oxidative stress resistance and root colonization by soybean rhizobia. Genomic and physiological analyses supported the fact that the mutation of a highly conserved valine ...

    • Rapid and cloning‑free screening of edited alfalfa via next‑generation sequencing 

      Stritzler, Margarita; Pascuan, Cecilia Gabriela; Bottero, Ana Emilia; Gomez, Maria Cristina; Frare, Romina Alejandra; Puebla, Andrea Fabiana; Tajima, Hiromi; Ayub, Nicolás Daniel; Blumwald, Eduardo; Soto, Gabriela Cynthia (Springer, 2022-07)
      The identification of full knockout plants is frequently the rate-limiting step of the research and commercial projects searching the complete loss of the function of target genes via the CRISPR/Cas9 system. This problem ...

    • Retrotransposon and CRISPR/Cas9‑mediated knockout of NOD26 impairs the legume‑rhizobia symbiosis 

      Frare, Romina Alejandra; Stritzler, Margarita; Gomez, Maria Cristina; Tajima, Hiromi; Pascuan, Cecilia Gabriela; López Fernández, María Paula; Bottero, Ana Emilia; Nikel, Pablo I.; Alleva, Karina Edith; Ayub, Nicolás Daniel; Blumwald, Eduardo; Soto, Gabriela Cynthia (Springer, 2022-07)
      The multifunctional channel NOD26, identified and extensively studied (both biochemically and biophysically) in soybean, is a major protein component of the symbiosome membrane. The water and ammonia transport activities ...

    • Stable symbiotic nitrogen fixation under water-deficit field conditions by a stress-tolerant alfalfa microsymbiont and its complete genome sequence : Short communication 

      Jozefkowicz, Cintia; Brambilla, Silvina Maricel; Frare, Romina Alejandra; Stritzler, Margarita; Piccinetti, Carlos Fabian; Puente, Mariana Laura; Berini, Carolina Andrea; Reyes Perez, Pedro Jose; Soto, Gabriela Cinthia; Ayub, Nicolás Daniel (2017-12)
      We here characterized the stress-tolerant alfalfa microsymbiont Sinorhizobium meliloti B401. B401-treated plants showed high nitrogen fixation rates under humid and semiarid environments. The production of glycine betaine ...

    • Synthetic multi-antibiotic resistant plasmids in plant-associated bacteria from agricultural soils 

      Brambilla, Silvina Maricel; Frare, Romina Alejandra; Stritzler, Margarita; Soto, Gabriela Cynthia; Berini, Carolina Andrea; Jozefkowicz, Cintia; Ayub, Nicolás Daniel (Elsevier, 2020-09)
      Objectives: Unlike higher organisms such as domestic animals and cultivated plants, which display a robust reproductive isolation and limited dispersal ability, microbes exhibit an extremely promiscuous gene flow and can ...

    • Whole-Genome Resequencing of Spontaneous Oxidative Stress-Resistant Mutants Reveals an Antioxidant System of Bradyrhizobium japonicum Involved in Soybean Colonization 

      Liebrenz, Karen Ivana; Gomez, Maria Cristina; Brambilla, Silvina Maricel; Frare, Romina Alejandra; Stritzler, Margarita; Maguire, Vanina; Ruiz, Oscar; Soldini, Diego Omar; Pascuan, Cecilia Gabriela; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (Springer, 2021-11)
      Soybean is the most inoculant-consuming crop in the world, carrying strains belonging to the extremely related species Bradyrhizobium japonicum and Bradyrhizobium diazoefficiens. Currently, it is well known that B. japonicum ...