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Comparative analysis of CRISPR/Cas9 delivery approaches for polyphenol oxidase 2 gene editing in potato
Gonzalez, Matías Nicolás; Massa, Gabriela Alejandra; Anderson, Mariette; Decima Oneto, Cecilia Andrea; Turesson, Helle; Storani, Leonardo; Olsson, Niklas; Fält, Ann Sofie; Hofvander, Per; Feingold, Sergio Enrique (Keystone Symposia on Molecular and Cellular Biology, 2021-03)StPPO2 gene editing was analyzed by Agrobacterium mediated transformation with CR-PPO vector, ribonucleoprotein complexes (RNP-PPO) transfection to protoplasts, and CR-PPO transient expression in protoplasts, yielding ... -
Comparative potato genome editing: Agrobacterium tumefaciens-mediated transformation and protoplasts transfection delivery of CRISPR/Cas9 components directed to StPPO2 gene
Gonzalez, Matías Nicolás; Massa, Gabriela Alejandra; Anderson, Mariette; Decima Oneto, Cecilia Andrea; Turesson, Helle; Olsson, Niklas; Fält, Ann Sofie; Hofvander, Per; Feingold, Sergio Enrique (Springer Netherlands, 2021-01-25)Delivery of the CRISPR/Cas9 components to the plant cells is a key step in its application as a genome editing tool. Here, we compared Agrobacterium-mediated transformation and protoplast transfection with CRISPR/Cas9 ... -
CRISPR/Cas9 for potato functional genomics and breeding
Gonzalez, Matías Nicolás; Massa, Gabriela Alejandra; Andersson, Mariette; Storani, Leonardo; Olsson, Niklas; Decima Oneto, Cecilia Andrea; Hofvander, Per; Feingold, Sergio Enrique (Humana Press (Springer), 2023-03-31)Cultivated potato (Solanum tuberosum L.) is one of the most important staple food crops worldwide. Its tetraploid and highly heterozygous nature pose a great challenge to its basic research and trait improvement through ... -
Genome-Wide Analyses of Aspartic Proteases on Potato Genome (Solanum tuberosum): Generating New Tools to Improve the Resistance of Plants to Abiotic Stress
Norero, Natalia Sigrid; Rey Burusco, María Florencia; D’Ippólito, Sebastián; Decima Oneto, Cecilia Andrea; Massa, Gabriela Alejandra; Castellote, Martín Alfredo; Feingold, Sergio Enrique; Guevara, María Gabriela (Multidisciplinary Digital Publishing Institute, MDPI, 2022-02)Aspartic proteases are proteolytic enzymes widely distributed in living organisms and viruses. Although they have been extensively studied in many plant species, they are poorly described in potatoes. The present study ... -
Identificación y caracterización de Aspartil proteasas en Solanum tuberosum relacionadas a diferentes tipos de estrés
Norero, Natalia Sigrid; Rey Burusco, Maria Florencia; D`Ippólito, S.; Castellote, Martín Alfredo; Decima Oneto, Cecilia Andrea; Massa, Gabriela Alejandra; Feingold, Sergio Enrique; Guevara, Gabriela (Secretaria de Ciencia y Tecnología, Universidad Nacional de Mar del Plata, 2020-10)En este trabajo, realizamos un análisis del genoma de S. tuberosum para explorar las funciones potenciales de las Aspartil proteasas (AP, EC 3.4.23). Las APs son enzimas proteolíticas con funciones muy diversas que se ... -
Obtención de plantas de papa sobrexpresantes de la aspartil proteasa StAP3 para incrementar la tolerancia a sequía
Decima Oneto, Cecilia Andrea; D`Ippólito, S.; Massa, Gabriela Alejandra; Rey, María Florencia; Norero, Natalia Sigrid; Feingold, Sergio Enrique; Guevara, Gabriela (Secretaria de Ciencia y Tecnología, Universidad Nacional de Mar del Plata, 2020-10)Una de las consecuencias del cambio climático es el estrés generado por la disminución de disponibilidad de agua (Leakey et al., 2009). La planta de papa, que representa una de las fuentes alimenticias más importantes para ... -
Reduced enzymatic browning in potato tubers by specific editing of a Polyphenol oxidase gene via Ribonucleoprotein complexes delivery of the CRISPR/Cas9 System.
Gonzalez, Matías Nicolás; Massa, Gabriela Alejandra; Andersson, Mariette; Turesson, Helle; Olsson, Niklas; Fält, Ann-Sofie; Storani, Leonardo; Decima Oneto, Cecilia Andrea; Hofvander, Per; Feingold, Sergio Enrique (Frontiers Media, Switzerland, 2020-01-09)Polyphenol Oxidases (PPOs) catalyze the conversion of phenolic substrates to quinones, leading to the formation of dark-colored precipitates in fruits and vegetables. This process, known as enzymatic browning, is the cause ... -
State of the art of genetic engineering in potato: from the first report to its future potential
Nahirñak, Vanesa; Almasia, Natalia Ines; Gonzalez, Matías Nicolás; Massa, Gabriela Alejandra; Decima Oneto, Cecilia Andrea; Feingold, Sergio Enrique; Hopp, Horacio Esteban; Vazquez Rovere, Cecilia (Frontiers Media, 2022-01)Potato (Solanum tuberosum L.) is a crop of world importance that produces tubers of high nutritional quality. It is considered one of the promising crops to overcome the challenges of poverty and hunger worldwide. However, ... -
Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought
Beznec, Ailin; Faccio, Paula Daniela; Miralles, Daniel J.; Abeledo, Leonor Gabriela; Decima Oneto, Cecilia Andrea; Garibotto, María De Belén; Gonzalez, Germán Andrés; Moreyra, Federico; Elizondo, Matias; Ruiz, Monica Beatriz; Lewi, Dalia Marcela; Blumwald, Eduardo; Llorente, Berta; Diaz Paleo, Antonio Horacio; Bossio, Adrian Ezequiel (Springer, 2021-05)Background: The heterologous expression of isopentenyl transferase (IPT) under the transcriptional control of the senescence-associated receptor-like kinase (SARK) promoter delayed cellular senescence and, through it, ... -
Transformación eficiente de Solanum tuberosum cv. Spunta mediada por Agrobacterium tumefaciens utilizando higromicina como agente selectivo
Decima Oneto, Cecilia Andrea; Coronel, Joel; Storani, Leonardo; Gonzalez, Matías Nicolás; Feingold, Sergio Enrique; Massa, Gabriela Alejandra (Ediciones INTA, 2020-08)La transformación genética de plantas es una herramienta biotecnológica de alto impacto en el mejoramiento vegetal que brinda la posibilidad de obtener nuevos cultivos que se adapten a las demandas de producción y de ... -
Validation of housekeeping genes for qPCR in maize during water deficit stress conditions at flowering time
Decima Oneto, Cecilia Andrea; Bossio, Adrian Ezequiel; Faccio, Paula Daniela; Beznec, Ailin; Blumwald, Eduardo; Lewi, Dalia Marcela (2017)Plant stress studies are increasingly being based on gene expression. The analysis of gene expression requires sensitive, precise, and replicable measurements for specific mRNA sequences. Real-time RT-PCR is nowadays the ... -
Water deficit stress tolerance in maize conferred by expression of an anisopentenyltransferase (IPT) gene driven by a stress- and maturation-induced promoter
Decima Oneto, Cecilia Andrea; Otegui, María Elena; Baroli, Irene; Beznec, Ailin; Faccio, Paula Daniela; Bossio, Adrian Ezequiel; Blumwald, Eduardo; Lewi, Dalia Marcela (2016)Senescence can be delayed in transgenic plants overexpressing the enzyme isopentenyltransferase (IPT) due to stress-induced increased levels of endogenous cytokinins. This trait leads to sustained photo-synthetic activity ...