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A new passive immune strategy based on IgY antibodies as a key element to control neonatal calf diarrhea in dairy farms
Vega, Celina Guadalupe; Bok, Marina; Ebinger, Maren; Rocha, Lucía Alejandra; Rivolta, Alejandra Antonella; González Thomas, Valeria; Muntadas, Pilar; D’Aloia, Ricardo; Pinto, Verónica; Parreño, Viviana; Wigdorovitz, Andres (BMC, 2020-07)Background: Neonatal diarrhea remains one of the main causes of morbi-mortality in dairy calves under artificial rearing. It is often caused by infectious agents of viral, bacterial, or parasitic origin. Cows vaccination ... -
Anti-VP6 VHH : an experimental treatment for rotavirus A-associated disease
Maffey, Lucia; Vega, Celina Guadalupe; Miño, Samuel; Garaicoechea, Lorena Laura; Parreño, Viviana (2016)Species A Rotaviruses (RVA) remain a leading cause of mortality in children under 5 years of age. Current treatment options are limited. We assessed the efficacy of two VP6-specific llama-derived heavy chain antibody ... -
Assessment of Influential Factors for Scours Associated with Cryptosporidium sp., Rotavirus and Coronavirus in Calves from Argentinean Dairy Farms
Bertoni, Emiliano Agustín; Barragán, Adrián A.; Bok, Marina; Vega, Celina Guadalupe; Martinez, Gabriela Marcela; Gil, José F.; Cimino, Rubén Oscar; Parreño, Gladys (MDPI, 2021-09)Scours is the most common disease in dairy calves, and it is a multifactorial syndrome complex. Cryptosporidium sp. (C. sp.), rotavirus group A (RVA), and bovine coronavirus (BCoV) are the three main pathogens associated ... -
Controlling Rotavirus-associated diarrhea: Could single-domain antibody fragments make the difference? = Hacia el control de la diarrea por rotavirus A: ¿podrían los nanoanticuerpos VHH marcar la diferencia?
Maffey, Lucia; Vega, Celina Guadalupe; Parreño, Viviana; Garaicoechea, Lorena Laura (Asociación Argentina de Microbiología, 2015-10)Group A Rotavirus (RVA) remains a leading cause of severe diarrhea and child mortality. The variable domain of camelid heavy chain antibodies (VHH) display potent antigen-binding capacity, have low production costs and are ... -
Development of a platform process for the production and purification of single‐domain antibodies
Crowell, Laura E.; Goodwine, Chaz; Sosa Holt, Carla Solange; Rocha, Lucía Alejandra; Vega, Celina Guadalupe; Rodriguez-Aponte, Sergio A.; Dalvie, Neil C.; Tracey, Mary Kate; Puntel, Mariana; Wigdorovitz, Andres; Parreño, Gladys; Love, Kerry R.; Cramer, Steven M.; Love, J. Christopher (Wiley, 2021-09)Single-domain antibodies (sdAbs) offer the affinity and therapeutic value of conventional antibodies, with increased stability and solubility. Unlike conventional antibodies, however, sdAbs do not benefit from a platform ... -
Development of an IgY-Based treatment to control bovine Coronavirus diarrhea in dairy calves
Bok, Marina; Vega, Celina Guadalupe; Castells, Matias; Colina, Rodney; Wigdorovitz, Andres; Parreño, Gladys Viviana (MDPI, 2023-03)Bovine Coronavirus (BCoV) is a major pathogen associated with neonatal calf diarrhea. Standard practice dictates that to prevent BCoV diarrhea, dams should be immunized in the last stage of pregnancy to increase BCoV-specific ... -
First characterization of a canine parvovirus causing fatal disease in coatis (Nasua nasua)
Bucafusco, Danilo; Argibay, Hernán Darío; Diaz, Leandro; Vega, Celina Guadalupe; Minatel, Leonardo; Postma, Gabriela C.; Rinas, Miguel; Bratanich, Ana Cristi (Springer, 2019-12)A canine parvovirus (CPV)-like virus was detected by PCR and isolated from dead coatis in Argentina. Analysis of the full-length genome sequence revealed that it resembled CPV-but also contained a mutation in the VP2 protein ... -
First report of group A rotavirus and bovine coronavirus associated with neonatal calf diarrhea in the northwest of Argentina
Bertoni, Emiliano Agustín; Aduriz Guerrero, Matías; Bok, Marina; Vega, Celina Guadalupe; Saif, Linda J.; Aguirre, Daniel Hector; Cimino, Rubén Oscar; Miño, Samuel; Parreño, Gladys Viviana (Springer, 2020-06)Group A rotavirus (RVA) and bovine coronavirus (BCoV) are the two main viral enteropathogens associated with neonatal calf diarrhea. The aim of the present survey was to investigate the epidemiology and the role of RVA and ... -
Influence of individual or group housing of newborn calves on rotavirus and coronavirus infection during the first 2 months of life
Bertoni, Emiliano Agustín; Bok, Marina; Vega, Celina Guadalupe; Martinez, Gabriela Marcela; Cimino, Rubén Oscar; Parreño, Gladys (Springer, 2021-01-03)Bovine rotavirus A (RVA) and bovine coronavirus (CoV) are the two main viral enteropathogens associated with neonatal calf diarrhea. The aim of the present work was to study the impact of group and individual housing systems ... -
Molecular and antigenic characterization of bovine Coronavirus circulating in Argentinean cattle during 1994–2010
Bok, Marina; Miño, Samuel; Rodriguez, Daniela; Badaracco, Alejandra; Nuñes, I.; Souza, Sibele P.; Bilbao, G.; Louge Uriarte, Enrique Leopoldo; Galarza, Roxana Ivon; Vega, Celina Guadalupe; Odeon, Anselmo Carlos; Saif, LInda J.; Parreño, Gladys Viviana (Elsevier, 2015-12)Bovine coronavirus (BCoV) is an important viral pathogen associated with neonatal calf diarrhea. Our aim was to investigate the incidence of BCoV in diarrhea outbreaks in beef and dairy herds from Argentina during 1994–2010. ... -
Molecular characterization of group A rotavirus strains detected in alpacas (Vicugna pacos) from Peru
Badaracco, Alejandra; Cordero, Aida; Vega, Celina Guadalupe; Bok, Marina; Zeller, Mark; Heylen, Elisabeth; Fernández, Gonzalo; Díez-Baños, Pablo; Morrondo, Patrocinio; Guevara, Héctor; Matthijnssens, Jelle; Parreño, Gladys Viviana (Microbiology Society, 2021-04)The alpaca is a very important social and economic resource for the production of fibre and meat for Andean communities. Peru is the main producer of alpacas. Group A rotavirus (RVA) has been sporadically detected in ... -
Molecular detection of bovine Noroviruses in Argentinean dairy calves : circulation of a tentative new genotype
Ferragut, Fátima; Vega, Celina Guadalupe; Mauroy, Axel; Conceição-Neto, Nádia; Zeller, Mark; Heylen, Elisabeth; Louge Uriarte, Enrique Leopoldo; Bilbao, Gladys; Bok, Marina; Matthijnssens, Jelle; Thiry, Etienne; Badaracco, Alejandra; Parreño, Gladys Viviana (2016)Bovine noroviruses are enteric pathogens detected in fecal samples of both diarrheic and non-diarrheic calves from several countries worldwide. However, epidemiological information regarding bovine noroviruses is still ... -
Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus
Dalvie, Neil C.; Brady, Joseph R.; Crowell, Laura E.; Tracey, Mary Kate; Biedermann, Andrew M.; Kaur, Kawaljit; Hickey, John M.; Kristensen II, D. Lee; Bonnyman, Alexandra D.; Rodriguez-Aponte, Sergio A.; Whittaker, Charles A.; Bok, Marina; Vega, Celina Guadalupe; Mukhopadhyay, Tarit K.; Joshi, Sangeeta B.; Volkin, David B.; Parreño, Gladys; Love, Kerry R.; Love, J. Christopher (BMC, 2021-05)Background: Vaccines comprising recombinant subunit proteins are well-suited to low-cost and high-volume production for global use. The design of manufacturing processes to produce subunit vaccines depends, however, on the ... -
Novel viruses determined using fecal virome analysis in the feces of foals with diarrea
Vega, Celina Guadalupe; Conceição-Neto, Nádia; Miño, Samuel; Zeller, Mark; Heylen, Elisabeth; Parreño, Viviana; Barrandeguy, Maria Edith; Matthijnssens, Jelle (2016)This study aims to expand the knowledge of viral species present in feces of foals suffering diarrhea using Next Generation Sequencing. This will be useful to further explore the role of new infectious agents in this illness ... -
Occurrence of Cryptosporidium and other enteropathogens and their association with diarrhea in dairy calves of Buenos Aires province, Argentina
Garro, Carlos Javier; Morici, Gabriel Edgardo; Tomazic, Mariela Luján; Vilte, Daniel Alejandro; Encinas, Micaela; Vega, Celina Guadalupe; Bok, Marina; Parreño, Viviana; Schnittger, Leonhard (Elsevier, 2021-04)Cryptosporidiosis of neonatal dairy calves causes diarrhea, resulting in important economic losses. In Argentina, prevalence values of Cryptosporidium spp. and other enteropathogens such as group A rotavirus (RVA), bovine ... -
Passive immunity to control Bovine coronavirus diarrhea in a dairy herd in Argentina = Inmunidad pasiva en el control de la diarrea por coronavirus bovino en un rodeo lechero de Argentina
Bok, Marina; Alassia, Martín; Frank, Flavia; Vega, Celina Guadalupe; Wigdorovitz, Andres; Parreño, Viviana (2018)Bovine coronavirus (BCoV) is a viral enteric pathogen associated with calf diarrhea worldwide being, in Argentina, mostly detected in dairy husbandry systems. The aim of the present work was to study if maternal IgG1 ... -
Prevención y tratamiento de Rotavirus A mediante nanoanticuerpos VHH anti-VP6
Maffey, Lucia (Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 2017-06)La diarrea asociada a Rotavirus A (RVA) representa una de las principales causas de mortalidad infantil a nivel mundial. Si bien la vacunación masiva contra RVA ha logrado disminuir significativamente las muertes causadas ...