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A dose-dependent response to MEK inhibition determines hypoblast fate in bovine embryos
Canizo, Jésica Romina; Ynsaurralde Rivolta, Amanda Eugenia; Vazquez Echegaray, Camila; Suvá, Mariana; Alberio, Virgilia; Aller Atucha, Juan Florencio; Guberman, Alejandra; Salamone, Daniel Felipe; Alberio, Ricardo; Alberio, Ramiro (BioMed Central, 2019-07-04)Background: The segregation of the hypoblast and the emergence of the pluripotent epiblast mark the final stages of blastocyst formation in mammalian embryos. In bovine embryos the formation of the hypoblast has been ... -
Effect of gaseous atmosphere on different supplementation medium for In Vitro canine oocyte maturation
González, Noelia; Gil, María Lydia; Rios, Glenda Laura; Canizo, Jésica Romina; De Blas, José Ignacio; Alberio, Ricardo (Bentham Open, 2014-02)The aim of the present research was to verify the influence of two different atmosphere gases (A: 5%CO2, B: 5%CO2, 5%O2 and 90%N2) on three different maturation media on in vitro maturation (IVM) rates for oocytes from ... -
Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system
Canizo, Jésica Romina; Vazquez Echegaray, Camila; Klisch, Doris; Aller Atucha, Juan Florencio; Paz, Dante; Alberio, Ricardo; Alberio, Ramiro; Guberman, Alejandra (BioMedCentral, 2018-07-27)OBJECTIVES: The use of induced pluripotent stem (iPS) cells as an alternative to embryonic stem cells to produce transgenic animals requires the development of a biotechnological platform for their generation. In this ... -
Free cholesterol and cholesterol esters in bovine oocytes: Implications in survival and membrane raft organization after cryopreservation
Buschiazzo, Jorgelina; Rios, Glenda Laura; Canizo, Jésica Romina; Antollini, Silvia Susana; Alberio, Ricardo (2017)Part of the damage caused by cryopreservation of mammalian oocytes occurs at the plasma membrane. The addition of cholesterol to cell membranes as a strategy to make it more tolerant to cryopreservation has been little ... -
In vitro characterization of anodized magnesium alloy as a potential biodegradable material for biomedical applications
Moreno, Julieta; Merlo, Julieta Leticia; Renno, Ana Claudia; Canizo, Jésica Romina; Buchelly, Francisco Javier; Pastore, Juan Ignacio; Katunar, Maria Rosa; Cere, Silvia (Elsevier, 2022-10)Pure metals and their alloys are used to partially or completely restore bone fractures. Biodegradable metals emerged as promising candidates for fracture fixation devices since they are able to self-degrade in the body ...