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A novel optimized polyoxometalate-modified silver nanocomposite. Application to the development of antibacterial textiles
Montemurro, Milagros; Enderle, Ana G.; Gutierrez, Fabiana; Lizarraga, Leonardo; Alvarez, Irene; Goicoechea, Héctor C.; Collins, Sebastián E.; Bollini, Mariela; Culzoni, María J. (Elsevier, 2023-06)Antimicrobial textiles are used in various settings ranging from households to different industries, health areas, and shops. These materials are used in the manufacture of air filters, food packaging, sportswear; and ... -
A safe and effective vaccine against bovine leukemia virus
Suarez Archilla, Guillermo; Gutierrez, Gerónimo; Camussone, Cecilia; Calvinho, Luis Fernando; Abdala, Alejandro Ariel; Alvarez, Irene; Petersen, Marcos; Franco, Lautaro Nahuel; De Stefano, Gabriel Alejandro; Monti, Gustavo; Jacques, Jean-Rock; Joris, Thomas; Willems, Luc; Trono, Karina Gabriela (Frontiers Media, 2022-08)Previous attempts to develop a vaccine against bovine leukemia virus (BLV) have not been successful because of inadequate or short-lived stimulation of all immunity components. In this study, we designed an approach based ... -
An indirect ELISA validated as diagnostic and vaccines quality control tool for bovine parainfluenza virus TYPE 3 (BPIV3)
Odeon, Maria Mercedes; Maidana, Silvina Soledad; Ferrecio, Carola Maria; Grazziotto, Noelia Magali; Pisano, Eddie; Alvarez, Irene; Rocha, Lucía Alejandra; Parreño, Gladys Viviana; Romera, Sonia Alejandra (CNRS (Francia), 2021-07)Serological assays, including enzyme-linked immunosorbent assays (ELISA), provide a useful tool to screen animals for the presence of antibodies (Abs) against a wide range of infectious agents (including viruses that cause ... -
Analysis of irradiated Argentinean fetal bovine serum for adventitious agents
Pecora, Andrea; Perez Lopez, Jorgelina; Jordan, Maximiliano Jesus; Franco, Lautaro Nahuel; Politzki, Romina Paula; Ruiz, Vanesa; Alvarez, Irene (Sage Publications, 2020-11)Fetal bovine serum (FBS) used in cell culture may be contaminated with adventitious agents, which can affect the production of biologicals and the results of clinical laboratory tests. We carried out a retrospective study ... -
BLV: lessons on vaccine development
Abdala, Alejandro Ariel; Alvarez, Irene; Brossel, Hélène; Calvinho, Luis Fernando; Carignano, Hugo; Franco, Lautaro Nahuel; Gazon, Hélène; Gillissen, Christelle; Hamaidia, Malik; Hoyos, Clotilde; Jacques, Jean-Rock; Joris, Thomas; Laval, Florent; Petersen, Marcos Iván; Porquet, Florent; Porta, Natalia Gabriela; Ruiz, Vanesa; Safari, Rogaiyeh; Suarez Archilla, Guillermo Alejandro; Trono, Karina Gabriela; Willems, Luc (BMC, 2019-10-07)Vaccination against retroviruses is a challenge because of their ability to stably integrate into the host genome, undergo long-term latency in a proportion of infected cells and thereby escape immune response. Since ... -
BOLA-DRB3 gene polymorphisms influence bovine leukaemia virus infection levels in Holstein and Holstein × Jersey crossbreed dairy cattle
Carignano, Hugo Adrian; Beribe, Maria Jose; Caffaro, María Eugenia; Amadio, Ariel; Nani, Juan Pablo; Gutierrez, Gerónimo; Alvarez, Irene; Trono, Karina Gabriela; Miretti, Marcos Mateo; Poli, Mario Andres (2017-05)Bovine leukemia virus (BLV) infections, causing persistent lymphocytosis and lethal lymphosarcoma in cattle, have reached high endemicity on dairy farms. We observed extensive inter-individual variation in the level of ... -
Bovine leukemia virus becomes established in dairy herds before the first lactation
Merlini, Ramiro; Gutierrez, Gerónimo; Alvarez, Irene; Jaworski, Juan Pablo; Carignano, Hugo Adrian; Poli, Mario Andres; Willems, Luc; Trono, Karina Gabriela (2016)In this work, we studied seven groups of pregnant heifers from a consortium of dairy farms heavily infected with bovine leukemia virus (BLV). ELISA testing showed that the seroprevalence ranges of BLV in heifers between ... -
Bovine leukemia virus infection in neonatal calves. Risk factors and control measures
Ruiz, Vanesa; Porta, Natalia Gabriela; Lomonaco, Marina; Trono, Karina Gabriela; Alvarez, Irene (Frontiers Media, 2018-10)Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL). Although efficient eradication programs have been successfully implemented in most European countries and Oceania, BLV infection rates ... -
Characterization of colostrum from dams of BLV endemic dairy herds
Gutierrez, Gerónimo; Lomonaco, Marina; Alvarez, Irene; Fernandez, Fernando; Trono, Karina Gabriela (Elsevier, 2015-06)Bovine Leukemia Virus (BLV) is endemic in Argentina, where the individual prevalence is higher than 80% in dairy farms. The aim of this work was to find preliminary evidence to know if the high level of infection of the ... -
Detección molecular de Parvovirus equino hepatitis en Argentina
Ruiz, Vanesa; Olguin Perglione, Cecilia; Alvarez, Irene (Universidad Nacional del Centro de la Provincia de Buenos Aires, 2023-08)El nuevo Parvovirus equino hepatitis EqPV H) identificado en 2018 está asociado con hepatitis subclínica a grave en caballos y parece ser el agente etiológico de la enfermedad de Theiler ( Esta enfermedad es una de las ... -
Detection of bovine leukemia virus RNA in blood samples of naturally infected dairy cattle
Alvarez, Irene; Porta, Natalia Gabriela; Trono, Karina Gabriela (MDPI, 2019-09)The viral expression in vivo, in bovine leukemia virus (BLV)-infected cattle, is considered to be restricted to extremely low levels, and the mitosis of infected B lymphocytes is regarded as the main mode of virus persistence ... -
Detection of equine infectious anemia virus by insulated isothermal RT-PCR (iiRT-PCR) assay using the POCKIT TM nucleic acid analyzer
Barrandeguy, Maria Edith; Espasandin, A.G.; Alvarez, Irene; Vissani, Maria Aldana; Martinez, D.E.; Chung, S.; Tsai, Y.; Lee, A.; Wang, T. (2016) -
Development of a droplet digital PCR assay for quantification of the proviral load of bovine leukemia virus
De Brun, María L.; Cosme, Bruno; Petersen, Marcos Iván; Alvarez, Irene; Folgueras-Flatschart, Aurea; Flatschart, Roberto; Panei, Carlos Javier; Puentes, Rodrigo (Sage Publications, 2022-05)Droplet digital PCR (ddPCR) is a highly sensitive tool developed for the detection and quantification of short-sequence variants—a tool that offers unparalleled precision enabling measurement of smaller-fold changes. We ... -
Diagnosis and control of Equine Infectious Anemia in a horse farm located in Buenos Aires province, Argentina
Vissani, Maria Aldana; Reynal O'Connor, J.; Olguin Perglione, Cecilia; Traverso, S.; Gutierrez, G.; Alvarez, Irene; Barrandeguy, Maria Edith (2016) -
Dynamics of perinatal bovine leukemia virus infection
Gutierrez, Gerónimo; Alvarez, Irene; Merlini, Ramiro; Rondelli, Flavia; Trono, Karina Gabriela (BioMed Central, 2014)Bovine leukemia virus (BLV) is highly endemic in many countries, including Argentina. As prevention of the spread from infected animals is of primary importance in breaking the cycle of BLV transmission, it is important ... -
The efficacy of ELISA commercial kits for the screening of equine infectious anemia virus infection = Eficacia de ensayos de ELISA comerciales para el screening de infección por el virus de la anemia infecciosa equina
Alvarez, Irene; Cipolini, Fabiana; Wigdorovitz, Andres; Trono, Karina Gabriela; Barrandeguy, Maria Edith (Elsevier España, 2015-03)The most used and reliable indicator of equine infectious anemia virus (EIAV) infection is the detection of its specific antibodies in horse serum. In the present study, the performance of two commercial ELISA tests for ... -
Efficacy of the spray-drying treatment to inactivate the bovine leukemia virus in bovine colostrum
Lomonaco, Marina; Sowul, Mariana; Gutierrez, Gerónimo; Malacari, Darío Amilcar; Alvarez, Irene; Porta, Natalia Gabriela; Zabal, Osvaldo Alfredo; Trono, Karina Gabriela (Elsevier, 2020-07)Previous studies have shown the presence of bovine leukemia virus (BLV) in colostrum and milk of naturally infected cows. The relationship between virus or provirus and specific antibodies in these secretions is particular ... -
Evaluación de la seguridad viral en productos biológicos
Salazar, Daniela; Franco, Lautaro Nahuel; Becerra, María Luciana; Perez Lopez, Jorgelina; Politzki, Romina Paula; Ruiz, Vanesa; Alvarez, Irene; Olguin Perglione, Cecilia (Universidad Nacional del Centro de la Provincia de Buenos Aires, 2023-08)El desarrollo de productos biofarmacéuticos requiere un control estricto de los procesos de manufactura a fin de obtener un producto seguro para humanos y animales Para esto es fundamental realizar el control de contaminantes ... -
Evaluation of total white blood cell count as a marker for proviral load of bovine leukemia virus in dairy cattle from herds with a high seroprevalence of antibodies against bovine leukemia virus
Alvarez, Irene; Gutierrez, Gerónimo; Gammella, Mariela; Martínez, Cecilia; Politzki, Romina Paula; González, Cintia Beatríz; Caviglia, Luciana; Carignano, Hugo; Fondevila, Norberto Antonio; Poli, Mario Andres; Trono, Karina Gabriela (2013-05)Objective—To determine the reference interval for WBC counts in Holstein dairy cows from herds with high seroprevalence for anti–bovine leukemia virus (BLV) antibodies, analyze the correlation of total WBC counts and blood ... -
Experimental infection of sheep with bovine leukemia virus (BLV): minimum dose of BLV-FLK cells and cell-free BLV and neutralization activity of natural antibodies = Infección experimental en ovinos con el virus de leucosis bovina: dosis mínima de células BLV-FLK y virus libre de células, y actividad neutralizante de anticuerpos naturales
Porta, Natalia Gabriela; Alvarez, Irene; Suarez Archilla, Guillermo Alejandro; Ruiz, Vanesa; Abdala, Alejandro Ariel; Trono, Karina Gabriela (Elsevier España, 2019-12)Bovine leukemia virus (BLV) is an important cattle pathogen that causes major economic losses worldwide, especially in dairy farms. The use of animal models provides valuable insight into the pathogenesis of viral infections. ...