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Cholesterol and desmosterol incorporation into ram sperm membrane before cryopreservation: Effects on membrane biophysical properties and sperm quality
Carro, María De Las Mercede; Peñalva, Daniel; Antollini, Silvia; Hozbor, Federico Andres; Buschiazzo, Jorgelina (Elsevier, 2020-09)Ram sperm are particularly sensitive to freeze-thawing mainly due to their lipid composition, limiting their use in artificial insemination programs. We evaluated the extent of cholesterol and desmosterol incorporation ... -
Combined epidermal growth factor and hyaluronic acid supplementation of in vitro maturation medium and its impact on bovine oocyte proteome and competence
Rios, Glenda Laura; Buschiazzo, Jorgelina; Mucci, Nicolas Crescencio; Kaiser, German Gustavo; Cesari, Andreina; Alberio, Ricardo (Elsevier, 2015-03-15)The conditions for in vitro oocyte maturation impact on cytoplasmic and nuclear processes in the oocyte. These events are differentially influenced by the nature of the maturation inducer and the presence of intact cumulus ... -
Dataset fron: Binding and fusion of Hoechst-labelled sperm to ZP-free oocytes matured in the presence of 43 µM or 100 µM LA (fresh and vitrified) [Conjunto de datos]
Rios, Glenda Laura; Suqueli García, María Florencia; Manrique, Rodrigo J.; Buschiazzo, Jorgelina (Biotecnología de la Reproducción - Laboratorio de Producción in vitro de Embriones - IPADS Balcarce (INTA-CONICET), 2025)The file contains the folowing columns : FRESH: non-vitrified oocytes. VITRIFIED: oocytes vitrified using a surface device (Cryotech®). CONTROL : oocytes matured without LA. 43 µM LA: oocytes matured in 43 µM LA. ... -
Desmosterol Incorporation Into Ram Sperm Membrane Before Cryopreservation Improves in vitro and in vivo Fertility
Carro, María de las Mercedes; Ramírez Vasquez, Rafael R.; Peñalva, Daniel A.; Buschiazzo, Jorgelina; Hozbor, Federico Andres (Frontiers Media, 2021-06)Pregnancy rates in ewes are markedly low after cervical insemination with frozen-thawed sperm. Sensitivity of ram sperm to freeze-thawing is related to the lipid composition of the membrane, particularly to its low sterol ... -
Free cholesterol and cholesterol esters in bovine oocytes: Implications in survival and membrane raft organization after cryopreservation
Buschiazzo, Jorgelina; Rios, Glenda Laura; Canizo, Jésica Romina; Antollini, Silvia Susana; Alberio, Ricardo (2017)Part of the damage caused by cryopreservation of mammalian oocytes occurs at the plasma membrane. The addition of cholesterol to cell membranes as a strategy to make it more tolerant to cryopreservation has been little ... -
Linoleic acid stimulates neutral lipid accumulation in lipid droplets of maturing bovine oocytes
Carro, María De Las Mercedes; Buschiazzo, Jorgelina; Rios, Glenda Laura; Oresti, Gerardo Martin; Alberio, Ricardo (Elsevier, 2013-03-01)Linoleic acid (LA) is a polyunsaturated fatty acid present in high concentrations in bovine follicular fluid; when added to maturation culture media, it affects oocyte competence (depending on the type and concentration ... -
Optimization of bovine oocyte cryopreservation: membrane fusion competence and cell death of linoleic acid-in vitro matured oocytes subjected to vitrification
Rios, Glenda Laura; Suqueli García, María Florencia; Manrique, Rodrigo J.; Buschiazzo, Jorgelina (Frontiers Media, 2025-07-03)Long-term conservation of livestock female genetic resources has led to increased interest in oocyte cryopreservation. However, the challenges of preserving large-volume cells and the lack of standardization in vitrification ... -
¿Por qué y para qué producir embriones bovinos in vitro?
Rios, Glenda Laura; Buschiazzo, Jorgelina (Estación Experimental Agropecuaria Balcarce, INTA, 2022-07)Nuevo récord mundial de embriones bovinos producidos in vitro promueve un giro hacia el uso de la criopreservación asociada al incremento sostenido en la producción de estos embriones. -
PUFA-rich phospholipid classes and subclasses of ram spermatozoa are unevenly affected by cryopreservation with a soybean lecithin-based extender
Carro, María De Las Mercedes; Luquez, J.M.; Peñalva, D.A.; Buschiazzo, Jorgelina; Hozbor, Federico Andres; Furland, N.E. (Elsevier, 2022-04)Cryopreservation is known to affect spermatozoa structure and function. Ram sperm are among the most highly sensitive mammalian gametes to freezing, due to their lipid composition, which limit their efficiency in artificial ...