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Analysis of management factors influencing Anaplasma marginale transmission
Morel, Nicolas; Mastropaolo, Mariano; Torioni, Susana Marta; Signorini Porchiett, Marcelo Lisandro; Mangold, Atilio Jose; Primo, María Evangelina (Elsevier, 2024-10)The aim of this study was to identify factors that play a key role in the epidemiology of bovine anaplasmosis by adapting a model primarily developed for cattle babesiosis. A cross-sectional observational study was conducted ... -
Assays to evaluate the transovarial transmission of Anaplasma marginale by Rhipicephalus microplus
Mazzucco Panizza, Matilde Nahimé; Cutulle, Christian; Primo, María Evangelina; Morel, Nicolas; Sebastian, Patrick; Nava, Santiago (Elsevier, 2022-11)The aim of this study was to evaluate the vectorial competence of Rhipicephalus microplus to transmit Anaplasma marginale transovarially, by analyzing the results of three different but complementary assays. First, larvae ... -
Comparison of real-time PCR and nested PCR based on the HlyA gene for the detection of Listeria monocytogenes. Application on cheese samples
Zbrun, Maria Virginia; Moreno, Nadia; Camussone, Cecilia; Signorini Porchiett, Marcelo Lisandro; Primo, María Evangelina (Springer, 2024-04)The aim of the present study was to compare the performance of a nested polymerase chain reaction (nPCR) and a real-time PCR based on the amplification of the HlyA gene from Listeria monocytogenes using a plasmid DNA ... -
Descripción de dos casos de babesiosis cerebral en terneros de hasta 15 días de edad
Olmos, Leandro Hipólito; Micheloud, Juan Francisco; Morel, Nicolas; Bertoni, Emiliano Agustín; Avellaneda-Cáceres, Agustín; Primo, María Evangelina; Thompson, Carolina Soledad; Torioni, Susana Marta; Aguirre, Daniel Hector (Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, 2020-06)La babesiosis es una enfermedad causada por Babesia bovis y Babesia bigemina, integrante del complejo conocido como “Tristeza bovina” y relevante en el Noroeste argentino (NOA). La presentación clínica de esta enfermedad ... -
Development and evaluation of a double-antigen sandwich ELISA to identify Anaplasma marginale–infected and A. centrale–vaccinated cattle
Sarli, Macarena; Thompson, Carolina Soledad; Novoa, María Belen; Valentini, Beatriz Susana; Mastropaolo, Mariano; Echaide, Ignacio Eduardo; Torioni, Susana Marta; Primo, María Evangelina (American Association of Veterinary Laboratory Diagnosticians, 2019-11)Bovine anaplasmosis is a worldwide infectious disease caused by the intraerythrocytic bacterium Anaplasma marginale, which is transmitted by ticks and fomites. A. centrale is a less virulent subspecies used as a live ... -
Development and field evaluation of a nested polymerase chain reaction-restriction fragment length polymorphism (nPCR-RFLP) analysis to identify A. marginale-infected and A. centrale-vaccinated cattle
Primo, María Evangelina; Bellezze, Julio; Morel, Nicolas; Mazzucco Panizza, Matilde Nahimé; Valentini, Beatriz Susana; Torioni, Susana Marta; Thompson, Carolina Soledad (Elsevier, 2022-07)A nested polymerase chain reaction-restriction fragment length polymorphism (nPCR-RFLP) assay based on the amplification of the Anaplasma spp. highly conserved msp5 gene and posterior digestion with HindIII endonuclease ... -
Development and field evaluation of an ELISA to differentiate Anaplasma marginale–infected from A. centrale–vaccinated cattle
Bellezze, Julio; Thompson, Carolina Soledad; Bosio, Anabela; Torioni, Susana Marta; Primo, María Evangelina (American Association of Veterinary Laboratory Diagnosticians, 2023-03)Immunization of calves with Anaplasma centrale is used to prevent acute anaplasmosis caused by A. marginale. Natural and vaccine-acquired immunity is detected through serologic tests based primarily on A. marginale recombinant ... -
Development of a novel fusion protein with Anaplasma marginale and A. centrale MSP5 improved performance of Anaplasma antibody detection by cELISA in infected and vaccinated cattle
Primo, María Evangelina; Thompson, Carolina Soledad; Valentini, Beatriz Susana; Sarli, Macarena; Novoa, María Belen; Mangold, Atilio Jose; Torioni, Susana Marta (Plos One, 2019-01-23)Detection of antibodies to Anaplasma spp. using commercial competitive enzyme-linked immunosorbent assay (ccELISA) is based on the recombinant major surface protein 5 fused to maltose binding protein (MBP-MSP5) or glutathione ... -
Development, validation and field evaluation of an indirect ELISA for the detection of antibodies against Brucella abortus in bulk and individual milk samples in dairy cattle
Novoa, María Belén; Aguirre, Nerina; Valentini, Beatriz Susana; Torioni, Susana Marta; Signorini Porchiett, Marcelo Lisandro; Primo, María Evangelina; Elena, Sebastián Alejandro; Vanzini, Victor Rene (Elsevier, 2022-11)Brucellosis is an abortigenic and zoonotic disease. In cattle, it is mainly caused by Brucella abortus. The disease is endemic in low- and middle-income countries, being considered a neglected zoonotic disease. In these ... -
Diagnóstico y prevención de la neosporosis bovina. Desarrollo de técnicas serológicas y evaluación de inmunógenos basados en proteínas recombinantes
Novoa, María Belén (Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, 2019)La neosporosis es una enfermedad abortígena, causada por el parásito protozoario Neospora caninum. No hay tratamientos o vacunas disponibles, el control se basa en el diagnóstico y las estrategias de manejo. Se desarrollaron ... -
Evaluation of a competitive inhibition ELISA based on the recombinant protein tSAG1 to detect anti–Neospora caninum antibodies in cattle
Novoa, María Belén; Valentini, Beatriz Susana; Torioni, Susana Marta; Primo, María Evangelina; Echaide, Ignacio Eduardo (American Association of Veterinary Laboratory Diagnosticians (AAVLD), 2020-05)Neospora caninum is a protozoan parasite that causes abortion and important economic losses in cattle worldwide. There are no treatments or vaccines available; disease control is based on diagnosis and herd management ... -
International interlaboratory validation of a nested PCR for molecular detection of Babesia bovis and Babesia bigemina, causative agents of bovine babesiosis
Ganzinelli, Sabrina Belen; Byaruhanga, Charles; Primo, María Evangelina; Lukanji, Zinathi; Sibeko, Kgomotso; Matjila, Tshepo; Neves, Luis; Benitez, Daniel Francisco; Enkhbaatar, Batmagnai; Nugraha, Arifin Budiman; Igarashi, Ikuo; Florin-Christensen, Mónica; Schnittger, Leonhard (Elsevier, 2022-04)Babesia bovis and B. bigemina are tick-transmitted parasites causing bovine babesiosis, characterized by significant morbidity and mortality leading to economic losses to the livestock industry in tropical and subtropical ... -
Manual para el abordaje diagnóstico de la babesiosis y anaplasmosis bovina en Argentina
Mazzucco Panizza, Matilde Nahimé; Morel, Nicolas; Wilkowsky, Silvina Elizabeth; Primo, María Evangelina; Olmos, Leandro Hipolito; Pertile, Carla Natalia; Sarmiento, Nestor Fabian; Rossner, Maria Victoria; Abdala, Alejandro Ariel; Canton, German Jose; Ganzinelli, Sabrina Belen; Thompson, Carolina Soledad; Guillemi, Eliana Carolina; Nava, Santiago (Ediciones INTA, 2024-02)La babesiosis y la anaplasmosis bovina son dos enfermedades infecciosas causantes de importantes pérdidas económicas para la producción ganadera en el centro y norte de la Argentina, principalmente en concepto de mortandad ... -
Molecular detection and phylogenetic analysis of Anaplasma platys-like and Candidatus Anaplasma boleense strains from Argentina
Sebastian, Patrick; Mazzucco Panizza, Matilde Nahimé; García Ríos, Ignacio José María; Tarragona, Evelina Luisa; Trova, Gabriela Beatriz; Sánchez Negrette, Olga; Primo, María Evangelina; Nava, Santiago (Elsevier, 2023-05)The present study aimed at the molecular detection of Anaplasma spp. in different samples obtained from cattle, goats and free-living Rhipicephalus microplus ticks from Argentina. DNA of members of the Anaplasmataceae ... -
Neospora caninum truncated recombinant proteins formulated with liposomes and CpG-ODNs triggered a humoral immune response in cattle after immunisation and challenge
Novoa, María Belén; Sarli, Macarena; Reidel, Ivana Gabriela; Veaute, Carolina Melania Isabel; Valentini, Beatriz Susana; Primo, María Evangelina (Elsevier, 2021-06)Abortions caused by Neospora caninum are a serious problem in cattle production and require effective immunoprophylaxis. The objective of this work was to assess the humoral immune response to four recombinant (r) N. caninum ... -
Producción y evaluación de antígenos recombinantes para el diagnóstico serológico y la generación de vacunas para el control de la anaplasmosis bovina
Sarli, Macarena (Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, 2020)La anaplasmosis bovina, causada por Anaplasma marginale (rickettsia intraeritrocítica transmitida por artrópodos hematófagos), es una enfermedad infecciosa de gran interés por las severas pérdidas económicas que ocasiona. ... -
Producción y evaluación de antígenos recombinantes para el diagnóstico serológico y la generación de vacunas para el control de la anaplasmosis bovina
Sarli, Macarena (Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, 2020)En este trabajo se abordó la anaplasmosis de forma integral, desde el diagnóstico y la prevención. La evaluación serológica de la enfermedad se realiza por ELISA de competición (ELISAc), utilizando como antígeno a la ... -
Sensitivity of Anaplasma marginale genotypes to oxytetracycline assessed by analyzing the msp1α gene in experimentally infected cattle
Primo, María Evangelina; Sarli, Macarena; Thompson, Carolina Soledad; Torioni, Susana Marta; Echaide, Ignacio Eduardo (Elsevier, 2021-09)The aim of this study was to evaluate the influence of the long-acting oxytetracycline (OTC) treatment on A. marginale genotypes of the isolate S1P, by analyzing the msp1α genotype based on a microsatellite (ms) and tandem ... -
Use and comparison of serologic assays to detect anti-Neospora caninum antibodies in farmed red deer (Cervus elaphus)
Novoa, María Belén; Soler, Juan Pablo; Cirone, Karina Mariela; Hecker, Yanina; Valentini, Beatriz Susana; Primo, María Evangelina; Moore, Prando Dadin (Elsevier, 2023-01)Neospora caninum is a protozoan parasite that cause abortion in different ruminant species, including red deer ( Cervus elaphus). There are no validated assays to be performed with sera from red deer. At the present work, ... -
A vaccine using Anaplasma marginale subdominant type IV secretion system recombinant proteins was not protective against a virulent challenge
Sarli, Macarena; Novoa, María Belen; Mazzucco Panizza, Matilde Nahimé; Signorini, Marcelo; Echaide, Ignacio Eduardo; Echaide, Susana T. de; Primo, María Evangelina (PLOS; National Center for Biotechnology Information, 2020-02)Anaplasma marginale is the most prevalent tick-borne livestock pathogen with worldwide distribution. Bovine anaplasmosis is a significant threat to cattle industry. Anaplasmosis outbreaks in endemic areas are prevented via ...