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Advances in novel vaccines for foot and mouth disease: focus on recombinant empty capsids
Mignaqui, Ana Clara; Ruiz, Vanesa; Durocher, Yves; Wigdorovitz, Andres (Taylor & Francis, 2019-05)Foot and mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals, which causes severe economic losses in the livestock industry. Currently available vaccines are based on inactivated FMD virus (FMDV). ... -
Analysis of irradiated Argentinean fetal bovine serum for adventitious agents
Pecora, Andrea; Perez Lopez, Jorgelina; Jordan, Maximiliano Jesus; Franco, Lautaro Nahuel; Politzki, Romina Paula; Ruiz, Vanesa; Alvarez, Irene (Sage Publications, 2020-11)Fetal bovine serum (FBS) used in cell culture may be contaminated with adventitious agents, which can affect the production of biologicals and the results of clinical laboratory tests. We carried out a retrospective study ... -
BLV: lessons on vaccine development
Abdala, Alejandro Ariel; Alvarez, Irene; Brossel, Hélène; Calvinho, Luis Fernando; Carignano, Hugo; Franco, Lautaro Nahuel; Gazon, Hélène; Gillissen, Christelle; Hamaidia, Malik; Hoyos, Clotilde; Jacques, Jean-Rock; Joris, Thomas; Laval, Florent; Petersen, Marcos Iván; Porquet, Florent; Porta, Natalia Gabriela; Ruiz, Vanesa; Safari, Rogaiyeh; Suarez Archilla, Guillermo Alejandro; Trono, Karina Gabriela; Willems, Luc (BMC, 2019-10-07)Vaccination against retroviruses is a challenge because of their ability to stably integrate into the host genome, undergo long-term latency in a proportion of infected cells and thereby escape immune response. Since ... -
Bovine dendritic cell activation, T cell proliferation and antibody responses to foot-and-mouth disease, is similar with inactivated virus and virus like particles
Quattrocchi, Valeria; Bidart, Juan Esteban; Mignaqui, Ana Clara; Ruiz, Vanesa; Ferella, Alejandra; Langellotti, Cecilia Ana; Gammella, Mariela; Ferraris, Sergio; Carrillo, Jorge Ernesto; Wigdorovitz, Andres; Durocher, Yves; Cardillo, Sabrina Beatriz; Charleston, Bryan; Zamorano, Patricia Ines (Frontiers Media, 2020-09)Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals that causes severe economic losses in the livestock industry. Currently available vaccines are based on the inactivated FMD virus (FMDV). ... -
Bovine leukemia virus infection in neonatal calves. Risk factors and control measures
Ruiz, Vanesa; Porta, Natalia Gabriela; Lomonaco, Marina; Trono, Karina Gabriela; Alvarez, Irene (Frontiers Media, 2018-10)Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL). Although efficient eradication programs have been successfully implemented in most European countries and Oceania, BLV infection rates ... -
Comparison of strategies for the production of FMDV empty capsids using the Baculovirus Vector System
Ruiz, Vanesa; Mignaqui, Ana Clara; Nuñez, M.C.; Reytor, Edel; Escribano, José M.; Wigdorovitz, Andres (Springer, 2014-11)Recombinant FMDV empty capsids have been produced in insect cells and larvae using the baculovirus expression system, although protein yield and efficiency of capsid assembly have been highly variable. In this work, two ... -
Comparison of transient and stable expression of foot-and-mouth disease virus capsid proteins in mammalian cells
Mignaqui, Ana Clara; Ruiz, Vanesa; Wigdorovitz, Andres (Scientific Research, 2013-12)Foot-and-mouth disease is a highly contagious disease that produces severe economic losses in the livestock industry. This disease is being controlled by the use of an inactivated vaccine. However, the use of recombinant ... -
Detección molecular de Parvovirus equino hepatitis en Argentina
Ruiz, Vanesa; Olguin Perglione, Cecilia; Alvarez, Irene (Universidad Nacional del Centro de la Provincia de Buenos Aires, 2023-08)El nuevo Parvovirus equino hepatitis EqPV H) identificado en 2018 está asociado con hepatitis subclínica a grave en caballos y parece ser el agente etiológico de la enfermedad de Theiler ( Esta enfermedad es una de las ... -
Evaluación de la seguridad viral en productos biológicos
Salazar, Daniela; Franco, Lautaro Nahuel; Becerra, María Luciana; Perez Lopez, Jorgelina; Politzki, Romina Paula; Ruiz, Vanesa; Alvarez, Irene; Olguin Perglione, Cecilia (Universidad Nacional del Centro de la Provincia de Buenos Aires, 2023-08)El desarrollo de productos biofarmacéuticos requiere un control estricto de los procesos de manufactura a fin de obtener un producto seguro para humanos y animales Para esto es fundamental realizar el control de contaminantes ... -
Experimental infection of sheep with bovine leukemia virus (BLV): minimum dose of BLV-FLK cells and cell-free BLV and neutralization activity of natural antibodies = Infección experimental en ovinos con el virus de leucosis bovina: dosis mínima de células BLV-FLK y virus libre de células, y actividad neutralizante de anticuerpos naturales
Porta, Natalia Gabriela; Alvarez, Irene; Suarez Archilla, Guillermo Alejandro; Ruiz, Vanesa; Abdala, Alejandro Ariel; Trono, Karina Gabriela (Elsevier España, 2019-12)Bovine leukemia virus (BLV) is an important cattle pathogen that causes major economic losses worldwide, especially in dairy farms. The use of animal models provides valuable insight into the pathogenesis of viral infections. ... -
First report of Equine Parvovirus-Hepatitis (EqPV-H) in Argentina
Olguin Perglione, Cecilia; Politzki, Romina Paula; Alvarez, Irene; Ruiz, Vanesa (Elsevier, 2024-07)The novel Equine Parvovirus-Hepatitis (EqPV-H) was first identified in the serum and liver of a horse that died of equine serum hepatitis, also known as Theiler's disease. Several reports in recent years strongly suggest ... -
Incidencia de la garrapata común del bovino en la transmisión del virus de la leucosis bovina
Martinez, Norberto Claudio; Nava, Santiago; Ruiz, Vanesa (Ediciones INTA; Estación Experimental Agropecuaria Reconquista, 2021-01)Este trabajo tiene como finalidad, determinar el rol de la larva de la garrapata común del bovino (Rhipicephalus microplus) en la transmisión del Virus de la Leucosis Bovina (VLB) de un animal enfermo a otro susceptible. ... -
Inter-laboratory comparison of eleven quantitative or digital PCR assays for detection of proviral bovine leukemia virus in blood samples
Pluta, Aneta; Jaworski, Juan Pablo; Droscha, Casey; VanderWeele, Sophie; Taxis, Tasia M.; Valas, Stephen; Brnić, Dragan; Jungić, Andreja; Ruano, María José; Sánchez, Azucena; Murakami, Kenji; Nakamura, Kurumi; Puentes, Rodrigo; De Brun, María L.; Ruiz, Vanesa; Ladera Gómez, Marla Eliana; Lendez, Pamela Anahí; Dolcini, Guillermina Laura; Fernandes Camargos, Marcelo; Fonseca, Antonio; Barua, Subarna; Wang, Chengming; Giza, Aleksandra; Kuźmak, Jacek (BioMed Central, 2024-08)Bovine leukemia virus (BLV) is the etiological agent of enzootic bovine leukosis and causes a persistent infection that can leave cattle with no symptoms. Many countries have been able to successfully eradicate BLV through ... -
Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice
Ruiz, Vanesa; Baztarrica, Josefina; Rybicki, Edward P.; Meyers, Ann E.; Wigdorovitz, Andres (Elsevier, 2018-12)Foot-and-mouth disease (FMD) remains one of the most feared viral diseases affecting cloven-hoofed animals, and results in severe economic losses. Currently available vaccines are based on inactivated FMD virus (FMDV). The ... -
Parvovirus Equino-Hepatitis (EqPV-H) : un nuevo virus contaminante de productos biológicos de origen veterinario = Equine Parvovirus-Hepatitis : A new adventitious agent in veterinary biological products
Ruiz, Vanesa; Alvarez, Irene (Facultad de Ciencias Veterinarias, Universidad Nacional del Nordeste, 2023-11)El Parvovirus Equino-Hepatitis (EqPV-H) es un nuevo integrante de la familia Parvoviridae descubierto hace apenas 5 años. Este virus fue identificado en el suero e hígado de un caballo muerto por hepatitis sérica equina, ... -
Plant‐produced viral bovine vaccines: what happened during the last 10 years?
Ruiz, Vanesa; Mozgovoj, Marina Valeria; Dus Santos, Maria Jose; Wigdorovitz, Andres (Wiley, 2015-10)Vaccination has proved to be an efficient strategy to deal with viral infections in both human and animal species. However, protection of cattle against viral infections is still a major concern in veterinary science. ... -
Primer reporte de ADN proviral del virus de la leucosis bovina detectado en carne destinada a consumo en Argentina
Porta, Natalia Gabriela; Ruiz, Vanesa (Universidad Nacional del Centro de la Provincia de Buenos Aires, 2023-08)Virus de la leucosis bovina (BLV). Familia: Retroviridae , Genero: Deltaretrovirus , Genoma: ssRNA. Agente causal de la Leucosis bovina enzoótica. Prevalencia predial: Tambos 80 99 Establecimientos de producción de carne ... -
Puesta a punto de una PCR en tiempo real para la detección de Senecavirus A
Franco, Lautaro Nahuel; Ruiz, Vanesa; Alvarez, Irene (Universidad Nacional del Centro de la Provincia de Buenos Aires, 2023-08)Senecavirus A (SVA). Familia : Picornaviridae Única especie del género Senecavirus. No envuelto ARN + monocatenario. La Organización Mundial de Sanidad Animal ( lo considera de gran importancia debido a que sus signos ... -
Secuencias parciales del gen NS1 de Parvovirus Equino-Hepatitis (EqPV-H) detectados en sueros equinos de Argentina [Conjunto de datos]
Ruiz, Vanesa; Olguin Perglione, Cecilia (Instituto Nacional de Tecnología Agropecuaria (INTA); Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), 2024)El set de datos corresponde a 12 secuencias parciales del gen NS1 de Parvovirus Equino-Hepatitis (EqPV-H). Estas secuencias fueron detectadas en sueros equinos provenientes de caballos donantes y de sueros equinos comerciales ... -
Seroprevalence and risk factors associated with bovine Leukemia virus infection in argentine beef cattle
Porta, Natalia Gabriela; Suarez Archilla, Guillermo; Miotti, Camila; Molineri, Ana Ines; Alvarez, Irene; Trono, Karina Gabriela; Signorini Porchiett, Marcelo Lisandro; Ruiz, Vanesa (Elsevier, 2023-11)Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis, an endemic disease in dairy cattle of Argentina. However, little is known about the seroprevalence of BLV in beef cattle. In this study, we ...