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A fijivirus major viroplasm protein shows RNA-stimulated ATPase activity by adopting pentameric and hexameric assemblies of dimers
Llauger, Gabriela; Melero, Roberto; Monti, Demian Esteban; Sycz, Gabriela; Huck-Iriart, Cristian; Cerutti, Maria Laura; Klinke, Sebastián; Mikkelsen, Evelyn; Tijman, Ariel; Arranz, Rocío; Alfonso, Victoria; Arellano, Sofía Maité; Goldbaum, Fernando Alberto; Sterckx, Yann G. J.; Carazo, José María; Kaufman, Sergio B.; Dans, Pablo D.; Del Vas, Mariana; Otero, Lisandro H. (American Society for Microbiology, 2023-02)Fijiviruses replicate and package their genomes within viroplasms in a pro cess involving RNA-RNA and RNA-protein interactions. Here, we demonstrate that the 24 C-terminal residues (C-arm) of the P9-1 major viroplasm protein ... -
AcMNPV Core Gene ac109 Is Required for Budded Virion Transport to the Nucleus and for Occlusion of Viral Progeny
Alfonso, Victoria; Maroniche, Guillermo Andrés; Reca, Sol Rita; Lopez, Maria Gabriela; Del Vas, Mariana; Taboga, Oscar Alberto (Plos One, 2012-09)The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac109 core gene has been previously characterized as an essential late gene. Our results showed that budded virions could be detected in supernatants of ... -
Baculovirus transduction in mammalian cells is affected by the production of type I and III interferons, which is mediated mainly by the cGAS-STING pathway
Amalfi, Sabrina; Molina, Guido Nicolas; Bevacqua, Romina Jimena; Lopez, Maria Gabriela; Taboga, Oscar Alberto; Alfonso, Victoria (American Society for Microbiology, 2020-11)The baculovirus Autographa californica multiple nucleopolyhedrovirus is an insect virus with a circular double-stranded DNA genome, which, among other multiple biotechnological applications, is used as an expression vector ... -
Biotechnological applications of occlusion bodies of Baculoviruses
Lopez, Maria Gabriela; Diez, Michay; Alfonso, Victoria; Taboga, Oscar Alberto (Springer, 2018-08)The ability of Baculoviruses to hyper-express very late genes as polyhedrin, the major component of occlusion bodies (OBs) or polyhedra, has allowed the evolution of a system of great utility for biotechnology. The main ... -
Comparative Pathogenesis of Generalist AcMNPV and Specific RanuNPV in Larvae of Rachiplusia nu (Lepidoptera: Noctuidae) Following Single and Mixed Inoculations
Decker Franco, Cecilia; Taibo, Catalina Beatriz; Di Rienzo, Julio A.; Alfonso, Victoria; Arneodo Larochette, Joel Demian (Oxford University Press, 2021-02-19)The South American soybean pest, Rachiplusia nu (Guenée), is naturally infected by Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and Rachiplusia nu nucleopolyhedrovirus (RanuNPV). We compared their pathogenicity ... -
Development of a novel set of Gateway-compatible vectors for live imaging in insect cells
Maroniche, Guillermo Andrés; Mongelli, Vanesa Claudia; Alfonso, Victoria; Llauger, Gabriela; Taboga, Oscar Alberto; Del Vas, Mariana (The Royal Entomological Society, 2011-10)Insect genomics is a growing area of research. To exploit fully the genomic data that are being generated, high-throughput systems for the functional characterization of insect proteins and their interactomes are required. ... -
Effects of deletion of the ac109 gene of Autographa californica nucleopolyhedrovirus on interactions with mammalian cells
Alfonso, Victoria; Amalfi, Sabrina; Lopez, Maria Gabriela; Taboga, Oscar Alberto (2017-03)Baculoviruses are able to enter into mammalian cells, where they can express a transgene that is placed under an appropriate promoter, without producing infectious progeny. ORF109 encodes an essential baculovirus protein ... -
Highly efficient production of rabies virus glycoprotein G ectodomain in Sf9 insect cells
Targovnik, Alexandra Marisa; Ferrari, Alejandro; Mc Callum, Gregorio Juan; Arregui, Mariana Bernadett; Smith, Ignacio; Bracco, Lautaro Fidel; Alfonso, Victoria; Lopez, Maria Gabriela; Martínez‑Solís, María; Herrero, Salvador; Miranda, Maria Victoria (Springer, 2019-11)In the present study, we developed a complete process to produce in insect cells a high amount of the ectodomain of rabies virus glycoprotein G (GE) as suitable antigen for detecting anti-rabies antibodies. Using the ... -
In vivo subcellular localization of Mal de Río Cuarto virus (MRCV) non-structural proteins in insect cells reveals their putative functions
Maroniche, Guillermo Andrés; Mongelli, Vanesa Claudia; Llauger, Gabriela; Alfonso, Victoria; Taboga, Oscar Alberto; Del Vas, Mariana (Elsevier, 2012-09)The in vivo subcellular localization of Mal de Río Cuarto virus (MRCV, Fijivirus, Reoviridae) non-structural proteins fused to GFP was analyzed by confocal microscopy. P5-1 showed a cytoplasmic vesicular-like distribution ... -
Interaction of Mal de Río Cuarto virus (Fijivirus genus) proteins and identification of putative factors determining viroplasm formation and decay
Llauger, Gabriela; De Haro, Luis Alejandro; Alfonso, Victoria; Del Vas, Mariana (2017)Mal de Río Cuarto virus (MRCV) is a member of the Fijivirus genus, within the Reoviridae family, that repli-cates and assembles in cytoplasmic inclusion bodies called viroplasms. In this study, we investigatedinteractions ... -
Novel biotechnological platform based on baculovirus occlusion bodies carrying Babesia bovis small antigenic peptides for the design of a diagnostic enzyme-linked immunosorbent assay (ELISA)
Lopez, Maria Gabriela; Pallares, H.M.; Alfonso, Victoria; Carmona, S. J.; Farber, Marisa Diana; Taboga, Oscar Alberto; Wilkowsky, Silvina Elizabeth (Springer, 2018-01)Baculoviruses are large DNA virus of insects principally employed in recombinant protein expression. Its ability to form occlusion bodies (OBs), which are composed mainly of polyhedrin protein (POLH), makes them ... -
P26 enhances baculovirus gene delivery by modulating the mammalian antiviral response
Amalfi, Sabrina; Plastine, María Del Pilar; Lopez, Maria Gabriela; Gravisaco, María José; Taboga, Oscar Alberto; Alfonso, Victoria (Springer, 2023-08)Poxins are poxviral proteins that act by degrading 2´3´-cGAMP, a key molecule of cGAS-STING axis that drives and amplifies the antiviral response. Previous works have described some poxin homologous among lepidopteran and ... -
Recombinant occlusion bodies of baculovirus as carriers of a nonstructural protein of foot-and-mouth disease virus
Diez, Michay; Trotta, Myrian Vanesa; Alfonso, Victoria; Taboga, Oscar Alberto; Lopez, Maria Gabriela (Springer, 2018-11)Here, we developed a diagnostic ELISA for foot-and-mouth disease using recombinant occlusion bodies (rOBs) of baculovirus. We fused Δ3AB1–3, a polypeptide derived from non-structural proteins of foot-and-mouth disease ... -
Surface Display of AcMNPV Occlusion-Derived P74 Does Not Enhance Oral Infectivity of Budded Viruses
Alfonso, Victoria; Lopez, Maria Gabriela; Carrillo, Elisa Cristina; Taboga, Oscar Alberto (S. Karger AG, 2012-02)Baculovirus occlusion-derived viruses (ODVs) and budded viruses (BVs) are morphologically and functionally distinct. ODVs are responsible for primary infection in insect hosts because of their high per os infectivity. On ... -
The autographa californica multiple nucleopolyhedrovirus Ac12: A non-essential F box-like protein that interacts with cellular SKP1 component of the E3 ubiquitin ligase complex
Costa Navarro, Guadalupe Soledad; Amalfi, Sabrina; Lopez, Maria Gabriela; Llauger, Gabriela; Arneodo Larochette, Joel Demian; Taboga, Oscar Alberto; Alfonso, Victoria (Elsevier, 2019-01)The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac12 gene, which is conserved in ten other baculovirus, codes a predicted 217 amino acid protein of unknown function. In this study, we investigated the ... -
VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display
Peralta, Andrea Veronica; Maroniche, Guillermo Andrés; Alfonso, Victoria; Molinari, Maria Paula; Taboga, Oscar Alberto (Elsevier, 2013-07)The Foot-and-mouth disease virus (FMDV) causes important economical losses in livestock farming. In order to develop a novel subunit vaccine against FMDV, we constructed recombinant baculoviruses that display the protein ...