autores
- xmlui.general.dspace_homexmlui.ArtifactBrowser.ConfigurableBrowse.trail.metadata.author
- Inicio
- autores
Autores
Mostrando ítems 1-20 de 27
-
A thermostable GH8 endoglucanase of Enterobacter sp. R1 is suitable for β- glucan deconstruction
Ontañon, Ornella Mailen; Ghio, Silvina; Marrero Diaz De Villegas, Rubén; Garrido, Mercedes Maria; Talia, Paola Mónica; Fehér, Csaba; Campos, Eleonora (Elsevier, 2019-11)Glycoside hydrolase family 8 (GH8) includes endoglucanases, lichenases, chitosanases and xylanases, which are essential for polysaccharides breakdown. In this work, we studied a thermally stable GH8 from the cellulose ... -
A thermostable laccase from Thermus sp. 2.9 and its potential for delignification of Eucalyptus biomass
Navas, Laura Emilce; Martinez, Fernando; Taverna, María Eugenia; Fetherolf, Morgan M.; Eltis, Lindsay D.; Nicolau, Veronica Viviana; Estenoz, Diana Alejandra; Campos, Eleonora; Benintende, Graciela Beatriz; Berretta, Marcelo Facundo (SpringerOpen, 2019-02)Laccases are multicopper oxidases that are being studied for their potential application in pretreatment strategies of lignocellulosic feedstocks for bioethanol production. Here, we report the expression and characterization ... -
Application of In-house xylanases as an addition to a commercial cellulase cocktail for the sustainable saccharification of pretreated blue agave bagasse used for bioethanol production
Montiel, Carmina; Hernández-Meléndez, Oscar; Marques, Susana; Gírio, Francisco; Tavares, João; Ontañon, Ornella Mailen; Campos, Eleonora; Bárzana, Eduardo (MDPI, 2024-08)The study involves the use of commercial cellulase Cellic CTec2 in combination with two in-house xylanases, PxXyn10A (XynA), a recombinant purified enzyme from Paenibacillus xylanivorans A59, and a xylanase enzymatic extract ... -
Characterization of a novel GH10 alkali‑thermostable xylanase from a termite microbiome
Mon, Maria Laura; Marrero Diaz De Vill, Rubén; Campos, Eleonora; Soria, Marcelo Abel; Talia, Paola Mónica (Springer Open, 2022-08)The aim of the present study was to assess the biochemical and molecular structural characteristics of a novel alkali-thermostable GH10 xylanase (Xyl10B) identified in a termite gut microbiome by a shotgun metagenomic ... -
Characterization of Cellulolytic Activities of Environmental Bacterial Consortia from an Argentinian Native Forest
Romano, Nelson Gaston; Gioffre, Andrea; Sede, Silvana Mabel; Campos, Eleonora; Cataldi, Angel Adrian; Talia, Paola Mónica (Springer, 2013-08)Cellulolytic activities of three bacterial consortia derived from a forest soil sample from Chaco region, Argentina, were characterized. The phylogenetic analysis of consortia revealed two main highly supported groups ... -
Characterization of two GH5 endoglucanases from termite microbiome using synthetic metagenomics
Ben Guerrero, Emiliano; Marrero Diaz De Villegas, Ruben; Soria, Marcelo Abel; Santangelo, María De La Paz; Campos, Eleonora; Talia, Paola Mónica (Springer, 2020-08)Here, we characterize two novel GH5 endoglucanases (GH5CelA and GH5CelB) from an uncultured bacterium identified in termite gut microbiomes. Both genes were codon-optimized, synthetized, cloned, and expressed as recombinant ... -
Draft Genome Sequence of Cellulolytic and Xylanolytic Cellulomonas sp. Strain B6 Isolated from Subtropical Forest Soil
Piccinni, Florencia Elizabeth; Murua, Yanina; Ghio, Silvina; Talia, Paola Mónica; Rivarola, Maximo Lisandro; Campos, Eleonora (2016-08)The genome information will be useful for studies of microbial enzymes for industrial application in lignocellulosic biomass utilization. -
EcXyl43 β-xylosidase: molecular modeling, activity on natural and artificial substrates, and synergism with endoxylanases for lignocellulose deconstruction
Ontañon, Ornella Mailén; Ghio, Silvina; Marrero Diaz De Vill, Rubén; Piccinni, Florencia Elizabeth; Talia, Paola Mónica; Cerutti, Maria Laura; Campos, Eleonora (Springer, 2018-08)Biomass hydrolysis constitutes a bottleneck for the biotransformation of lignocellulosic residues into bioethanol and high-value products. The efficient deconstruction of polysaccharides to fermentable sugars requires ... -
Evaluation of hydrothermal and alkaline pretreatment routes for xylooligosaccharides production from sugar cane bagasse using different combinations of recombinant enzymes
Capetti, Caio; Arnoldi Pellegrini, Vanessa; Vacilotto, Milena Moreira; da Silva Curvelo, Antonio Aprigio; Falvo, Maurício; Guimaraes, Francisco E.G.; Ontañon, Ornella Mailen; Campos, Eleonora; Polikarpov, Igor (Springer, 2023-10)Xylan is the most abundant constituent of hemicellulose fraction of lignocellulosic biomass. Short xylooligosaccharides (XOS), obtained via xylan hydrolysis, have well-known prebiotic and antioxidant properties that are ... -
GH10 XynA is the main xylanase identified in the crude enzymatic extract of Paenibacillus sp. A59 when grown on xylan or lignocellulosic biomass
Ghio, Silvina; Insani, Ester Marina; Piccinni, Florencia Elizabeth; Talia, Paola Mónica; Grasso, Daniel Horacio; Campos, Eleonora (2016)A novel bacterial isolate with polysaccharides degrading activity was identified as Paenibacillus sp., andnamed Paenibacillus sp. A59. Even though it is a strict mesophile, optimal xylanase activity of the crudeenzymatic ... -
Heterologous production and characterization of a thermostable GH10 family endo-xylanase from Pycnoporus sanguineus BAFC 2126
Niderhaus, Cecilia; Garrido, Mercedes Maria; Insani, Ester Marina; Campos, Eleonora; Wirth, Sonia Alejandra (Elsevier, 2018-04)Xylanases are key enzymes for agricultural biomass saccharification for the production of cellulosic ethanol. Success in enzymatic lignocellulose bioconversion is restricted by enzyme production costs, activity and stability ... -
Insights into the xylan degradation system of Cellulomonas sp. B6 : biochemical characterization of rCsXyn10A and rCsAbf62A
Garrido, Mercedes Maria; Piccinni, Florencia Elizabeth; Landoni, Malena; Peña, María Jesús; Topalian, Juliana; Couto, Alicia; Wirth, Sonia Alejandra; Urbanowicz, Breeanna Rae; Campos, Eleonora (Springer, 2022)Valorization of the hemicellulose fraction of plant biomass is crucial for the sustainability of lignocellulosic biorefineries. The Cellulomonas genus comprises Gram-positive Actinobacteria that degrade cellulose and other ... -
Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
Romero Victorica, Matias; Soria, Marcelo Abel; Batista-García, Ramón Alberto; Ceja-Navarro, Javier A.; Vikram, Surendra; Ortiz, Maximiliano; Ontañon, Ornella; Ghio, Silvina; Martínez-Ávila, Liliana; Quintero García, Omar Jasiel; Etcheverry, Clara; Campos, Eleonora; Cowan, Donald; Arneodo Larochette, Joel Demian; Talia, Paola Mónica (Springer Nature, 2020-03-02)In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different ... -
Obtención de mutantes de Brucella abortus genéticamente definidas: su caracteización molecular y evaluación de su uso como vacunas para la brucelosis bovina
Campos, Eleonora (Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 2017)El desarrollo de las técnicas de ADN recombinante permitió la generación de vacunas racionalmente diseñadas. Teniendo esto en cuenta, el objetivo de este trabajo es la obtención de cepas de Brucella abortus genéticamente ... -
Optimisation of xylanases production by two Cellulomonas strains and their use for biomass deconstruction
Ontañon, Ornella Mailen; Bedő, Soma; Ghio, Silvina; Garrido, Mercedes Maria; Topalian, Juliana; Jahola, Dóra; Fehér, Anikó; Valacco, Maria Pia; Campos, Eleonora; Fehér, Csaba (Springer, 2021-05)One of the main distinguishing features of bacteria belonging to the Cellulomonas genus is their ability to secrete multiple polysaccharide degrading enzymes. However, their application in biomass deconstruction still ... -
Optimization of cellobiohydrolase production and secretome analysis of Trametes villosa LBM 033 suitable for lignocellulosic bioconversion
Coniglio, Romina O.; Fonseca, María I.; Diaz, Gabriela V.; Ontañon, Ornella Mailén; Ghio, Silvina; Campos, Eleonora; Zapata, Pedro Darío (Taylor & Francis, 2019-04)The production of bioethanol from lignocellulosic biomass comprises the enzymatic hydrolysis of lignocellulosic structures by three major cellulases. Among them, cellobiohydrolases are considered to be key enzymes playing ... -
Paenibacillus sp. A59 GH10 and GH11 extracellular endoxylanases: application in biomass bioconversion
Ghio, Silvina; Ontañon, Ornella Mailén; Piccinni, Florencia Elizabeth; Marrero Diaz De Vill, Rubén; Talia, Paola Mónica; Grasso, Daniel Horacio; Campos, Eleonora (Springer, 2018-03)The cost-efficient degradation of xylan to fermentable sugars is of particular interest in second generation bioethanol production, feed, food, and pulp and paper industries. Multiple potentially secreted enzymes involved ... -
Paenibacillus xylanivorans sp. nov., a xylan-degrading bacterium isolated from decaying forest soil
Ghio, Silvina; Sauka, Diego Hernan; Ferrari, Alejandro E.; Piccinni, Florencia Elizabeth; Ontañon, Ornella Mailén; Campos, Eleonora (Microbiology Society, 2019-12)A xylanolytic bacterial strain, named A59T, was isolated from a forest soil consortium in southern Argentina. Strain A59T is a Gram-stain-positive, facultative anaerobic, endospore-forming and rod-shaped bacterium. Its ... -
Production of a bacterial secretome highly efficient for the deconstruction of xylans
Topalian, Juliana; Navas, Laura Emilce; Ontañon, Ornella Mailen; Valacco, Maria Pia; Noseda, Diego Gabriel; Blasco, Martín; Peña, María Jesús; Urbanowicz, Breeanna Rae; Campos, Eleonora (Springer, 2024-09)Bacteria within the Paenibacillus genus are known to secrete a diverse array of enzymes capable of breaking down plant cell wall polysaccharides. We studied the extracellular xylanolytic activity of Paenibacillus xylanivorans ... -
Prospección y caracterización de bacterias celulolíticas y hemicelulolíticas
Ghio, Silvina (Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 2018)La prospección de bacterias con capacidad de degradar lignocelulosa permite la identificación de nuevas enzimas con potencial aplicación en numerosos procesos industriales. En este trabajo el objetivo fue la obtención de ...