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An isopentenyl transferase transgenic wheat isoline exhibits less seminal root growth impairment and a differential metabolite profile under Cd stress
Gomez Mansur, Nabila M.; Pena, Liliana Beatriz; Bossio, Adrian Ezequiel; Lewi, Dalia Marcela; Beznec, Ailin; Blumwald, Eduardo; Arbona, Vicent; Gómez-Cadenas, Aurelio; Benavides, María Patricia; Gallego, Susana Mabel (Wiley, 2021-09)Cadmium is one of the most important contaminants and it induces severe plant growth restriction. In this study, we analyzed the metabolic changes associated with root growth restriction caused by cadmium in the early ... -
Aplicación de mutagénesis in vitro en genotipos INTA de caña de azúcar para la generación de variabilidad genética
Di Pauli, Valentina (Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, 2020-12)El mejoramiento genético de caña azúcar se enfrenta a su complejo genoma, la estrecha base genética y la baja fertilidad de los cultivares modernos que dificultan la obtención de genotipos superiores. La mutagénesis in ... -
Argentina’s local crop biotechnology developments: why have they not reached the market yet?
Lewi, Dalia Marcela; Vicien, Carmen (Frontiers Media, 2020-04)Plant biotechnology in Argentina started at the end of the 1980s, leading to the development of numerous research groups in public institutions and, a decade later, to some local private initiatives. The numerous scientific ... -
Development of a construct-based risk assessment framework for genetic engineered crops
Becker, M.P.; Boari, P.; Burachik, Moisés; Cuadrado, V.; Junco, Mariano; Lede, S.; Lema, Martín; Lewi, Dalia Marcela; Maggi, Andrés; Meoniz,Ignacio Alberto; Noe, G.; Roca, Cecilia; Robredo, Claudio Gabriel; Rubinstein, Clara; Vicien, Carmen; Whelan, Agustina (2016)Experience gained in the risk assessment (RA) of genetically engineered (GE) crops since their first experimental introductions in the early nineties, has increased the level of familiarity with these breeding methodologies ... -
Efecto del etil metanosulfonato sobre la capacidad embriogénica y la regeneración in vitro en caña de azúcar
Di Pauli, Valentina; Fontana, Paola Daniela; Lewi, Dalia Marcela; Erazzu, Luis Ernesto (Asociación Latinoamericana de Genética, 2019)El mejoramiento genético de caña de azúcar se enfrenta a su complejo genoma, la estrecha base genética, y la fertilidad deficiente que dificultan la obtención de genotipos superiores. -
Estimación de la variabilidad genética generada en poblaciones mutagenizadas de caña de azúcar
Di Pauli, Valentina; Fontana, Paola Daniela; Lewi, Dalia Marcela; Felipe, Arturo; Erazzu, Luis Ernesto (REDBIO, 2021-06)La mutagénesis in vitro se ha convertido en una herramienta útil en el desarrollo de mutantes, especialmente en plantas de propagación vegetativa, combinando la variación somaclonal generada por el cultivo in vitro y la ... -
Estudios regulatorios para las evaluaciones de riesgo de los cultivos transgénicos : un análisis preliminar
Vicien, Carmen; Lewi, Dalia Marcela (Facultad de Agronomía, Universidad de Buenos Aires, 2022-06)Los desarrollos tecnológicos vinculados con los organismos genéticamente modificados (OGM) hicieron necesaria la implementación de sistemas regulatorios destinados a garantizar la seguridad ambiental y la aptitud alimentaria. ... -
Evaluación in vitro de materiales de algodón Gossypium hirsutum L. en relación a la capacidad de regeneración y respuesta a estrés abiótico. Análisis de variedades comerciales de INTA, líneas avanzadas
Gonzalez, Ariela Judith (Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, 2015)El algodón juega un rol crucial en la economía global, ya sea a nivel social como en la industria de manufactura. Dado que se necesita contar con genotipos de alta calidad agronómica, capaces de regenerar in vitro, y con ... -
Impacto de la disrupción de la membrana celular mediada por Bacillus thuringiensis en la entrada de ARN interferente en Spodoptera frugiperda
Niz, José María; Lewi, Dalia Marcela; Hopp, Horacio Esteban; Salvador, Ricardo; Sauka, Diego Hernan (Asociación Argentina de Microbiología, 2024-08-21)El control de Spodoptera frugiperda, una de las plagas principales del maíz, es mediante insecticidas químicos y plantas transgénicas productoras de toxinas de Bacillus thuringiensis. Esta bacteria produce cristales que ... -
Optimized somatic embryogenesis and plant regeneration in elite Argentinian sugarcane (Saccharum spp.) cultivars
Di Pauli, Valentina; Fontana, Paola Daniela; Lewi, Dalia Marcela; Felipe, Arturo; Erazzu, Luis Ernesto (Springer, 2021-11)Background: Biotechnological breeding of elite sugarcane cultivars is currently limited because of the difficulty of regenerating plants by tissue culture. Here, we report that commercially elite sugarcane genotypes, which ... -
RNAi Expression in Cotton for Control of Herbivorous Insects
Salvador, Ricardo; Maskin, Laura; Niz, José María; Turica, Mariana Daniela; Pedarros, Analía Soledad; Hopp, Horacio Esteban; Lewi, Dalia Marcela (Springer, 2022)Cultivated cotton (Gossypium hirsutum) is heavily attacked by various species of insects worldwide and breeding of new varieties resistant to pests is still a hard battle to win. RNAi technology is an important reverse ... -
Science based criteria for a simplified risk assessment of genetic engineered crops developed using identical or similar constructs
Burachik, Moisés; Cuadrado, V.; Herrmann, Claudia; Junco, Mariano; Lede, S.; Lewi, Dalia Marcela; Maggi, Andrés; Meoniz, Ignacio Alberto; Noe, G.; Roca, Cecilia; Robredo, Claudio Gabriel; Rubinstein, Clara; Vicien, Carmen; Boari, P.; Lema, Martín; Whelan, Agustina (2016-08)Experience with Agricultural Biotechnology and the widespread adoption of different genetically engineered (GE) crops around the world has enabled breeders to develop different crops with similar phenotypic characteristics ... -
Shortening of generation cycles in inbred lines of maize (Zea mays L.) through embryo rescue technique
Liotino, Micaela; Varangot, Agostina; Beznec, Aylin; Auteri, Micol; Bossio, Adrian Ezequiel; Lewi, Dalia Marcela; Faccio, Paula Daniela (Istituto Sperimentale per la Cerealicoltura, 2019-11)Embryo rescue techniques have been used as an approach to raise hybrids from incompatible crosses. These techniques have also proved to be valuable tools for maize improvement, since they allow reducing the duration of the ... -
Somatic embryogenesis response in Argentinian sugarcane genotypes for in vitro mutagenesis application
Di Pauli, Valentina; Fontana, Paola Daniela; Lewi, Dalia Marcela; Felipe, Arturo; Erazzu, Luis Ernesto (International Society of Sugar Cane Technologists, 2019-09)Somatic embryogenesis is an excellent process for chemical mutation induction as somatic embryos originate from a single cell, hence limiting chimeras to very low frequencies in regenerated mutants. Callus formation and ... -
Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought
Beznec, Ailin; Faccio, Paula Daniela; Miralles, Daniel J.; Abeledo, Leonor Gabriela; Decima Oneto, Cecilia Andrea; Garibotto, María De Belén; Gonzalez, Germán Andrés; Moreyra, Federico; Elizondo, Matias; Ruiz, Monica Beatriz; Lewi, Dalia Marcela; Blumwald, Eduardo; Llorente, Berta; Diaz Paleo, Antonio Horacio; Bossio, Adrian Ezequiel (Springer, 2021-05)Background: The heterologous expression of isopentenyl transferase (IPT) under the transcriptional control of the senescence-associated receptor-like kinase (SARK) promoter delayed cellular senescence and, through it, ... -
Validation of housekeeping genes for qPCR in maize during water deficit stress conditions at flowering time
Decima Oneto, Cecilia Andrea; Bossio, Adrian Ezequiel; Faccio, Paula Daniela; Beznec, Ailin; Blumwald, Eduardo; Lewi, Dalia Marcela (2017)Plant stress studies are increasingly being based on gene expression. The analysis of gene expression requires sensitive, precise, and replicable measurements for specific mRNA sequences. Real-time RT-PCR is nowadays the ... -
Water deficit stress tolerance in maize conferred by expression of an anisopentenyltransferase (IPT) gene driven by a stress- and maturation-induced promoter
Decima Oneto, Cecilia Andrea; Otegui, María Elena; Baroli, Irene; Beznec, Ailin; Faccio, Paula Daniela; Bossio, Adrian Ezequiel; Blumwald, Eduardo; Lewi, Dalia Marcela (2016)Senescence can be delayed in transgenic plants overexpressing the enzyme isopentenyltransferase (IPT) due to stress-induced increased levels of endogenous cytokinins. This trait leads to sustained photo-synthetic activity ...