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Analysis of a chitinase from EpapGV, a fast killing betabaculovirus
Salvador, Ricardo; Ferrelli, Maria Leticia; Sciocco, Alicia Ines; Romanowski, Victor (Springer, 2014-04)The main function of baculoviral chitinase protein (V-CHIA) is to promote the final liquefaction of infected host larvae, facilitating the dispersion of occlusion bodies (OBs) in the environment. In this study, a v-chiA ... -
Baculovirus Insecticides in Latin America: Historical Overview, Current Status and Future Perspectives
Haase, Santiago; Sciocco, Alicia Ines; Romanowski, Victor (2015)Baculoviruses are known to regulate many insect populations in nature. Their host-specificity is very high, usually restricted to a single or a few closely related insect species. They are amongst the safest pesticides, ... -
Biological and molecular characterization of Rachiplusia nu single nucleopolyhedrovirus, a promising biocontrol agent against the South American soybean pest Rachiplusia nu
Jakubowicz, Violeta; Taibo, Catalina Beatriz; Sciocco, Alicia Ines; Arneodo Larochette, Joel Demian (Elsevier, 2019-09)Rachiplusia nu (Lepidoptera: Noctuidae) is a key soybean pest in Argentina. Current management of this moth relies mainly on the use of synthetic insecticides and transgenic plants. In search of biological control-based ... -
Biological and molecular features of Nosema rachiplusiae sp. n., a microsporidium isolated from the neotropical moth Rachiplusia nu (Guenée) (Lepidoptera: Noctuidae)
Arneodo Larochette, Joel Demian; Sciocco, Alicia Ines (Springer, 2018-05)Light, electron microscopy and DNA analyses were performed to characterize a microsporidium infecting Rachiplusia nu larvae from a laboratory rearing in Argentina. Diplokaryotic spores were oval and measured 3.61 ± 0.29 ... -
Characterization of a new Helicoverpa armigera nucleopolyhedrovirus variant causing epizootic on a previously unreported host, Helicoverpa gelotopoeon (Lepidoptera: Noctuidae)
Ferrelli, Maria Leticia; Taibo, Catalina Beatriz; Fichetti, Patricia; Sciocco, Alicia Ines; Arneodo Larochette, Joel Demian (2016)This paper reports the first biological and molecular characterization of a nucleopolyhedrovirus isolated from the soybean and cotton pest Helicoverpa gelotopoeon. Studies were performed following a virus outbreak in a ... -
Development of a Recombination System for the Generation of Occlusion Positive Genetically Modified Anticarsia Gemmatalis Multiple Nucleopolyhedrovirus
Haase, Santiago; McCarthy, Christina; Ferrelli, Maria Leticia; Pidre, Matias Luis; Sciocco, Alicia Ines; Romanowski, Victor (2015)Anticarsia gemmatalis is an important pest in legume crops in South America and it has been successfully controlled using Anticarsia gemmatalis Multiple Nucleopolyhedrovirus(AgMNPV) in subtropical climate zones. Nevertheless, ... -
Effect of the interaction between Anticarsia gemmatalis multiple nucleopolyhedrovirus and Epinotia aporema granulovirus, on A. gemmatalis (Lepidoptera: Noctuidae) larvae
Biedma, Marina Elizabeth; Salvador, Ricardo; Ferrelli, Maria Leticia; Sciocco, Alicia Ines; Romanowski, Víctor (Elsevier, 2015-12)The bean shoot borer Epinotia aporema Wals. (Lepidoptera: Tortricidae) and the velvet bean caterpillar Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae) are key pests of soybean and other legume crops in South America. ... -
Functional analysis of Spodoptera frugiperda nucleopolyhedrovirus late expression factors in Sf9 cells
Berretta, Marcelo Facundo; Lopez, Maria Gabriela; Taboga, Oscar Alberto; Sciocco, Alicia Ines; Romanowski, Victor (2013-02)We used transient expression assays to assess the function of the baculovirus Spodoptera frugiperda M nucleopolyhedrovirus (SfMNPV) homologs of Autographa californica MNPV (AcMNPV) factors involved in late gene expression ... -
Genetic variants in Argentinean isolates of Spodoptera frugiperda Multiple Nucleopolyhedrovirus
Niz, José María; Salvador, Ricardo; Ferrelli, María Leticia; Sciocco, Alicia Ines; Romanowski, Víctor; Berretta, Marcelo Facundo (Springer, 2020)The fall armyworm, Spodoptera frugiperda (JE Smith) is a key pest in the Americas. Control strategies are mainly carried out by use of chemical insecticides and transgenic crops expressing Bacillus thuringiensis toxins. ... -
Genomic analysis of an Argentinean isolate of Spodoptera frugiperda granulovirus reveals that various baculoviruses code for Lef-7 proteins with three F-box domains
Ferrelli, Maria Leticia; Pidre, Matias Luis; Ghiringhelli, Pablo Daniel; Torres, Sofía; Fabre, María Laura; Masson, Tomás; Cédola, Maia Tatiana; Sciocco, Alicia Ines; Romanowski, Victor (2018)A new isolate of the Spodoptera frugiperda granulovirus, SfGV ARG, was completely sequenced and analyzed. The SfGV ARG genome is 139,812 bp long and encodes 151 putative open reading frames. Of these ORFs, 56 were found ... -
Light microscopy of microsporidian spores and baculovirus occlusion bodies in Epinotia aporema and Helicoverpa gelotopoeon larval tissues, respectively
Arneodo Larochette, Joel Demian; Taibo, Catalina Beatriz; Sciocco, Alicia Ines (2016)Entomopathogens help regulate natural insect populations, and their use in pest management is the subject of active research. Classical and molecular techniques are currently available to elucidate disease etiology and ... -
Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in Argentina
Arneodo Larochette, Joel Demian; De Anna, J.; Salvador, Ricardo; Farinon, Omar Marcelo; Quintana, Graciela Mabel; Sciocco, Alicia Ines (2015-01)The occurrence and genetic diversity of Cydia pomonella granulovirus infecting C. pomonella across Argentina were investigated. Larvae were collected from fruit orchards in regions where CpGV‐based biopesticides have been ... -
Transcriptomic Survey of the Midgut of Anthonomus grandis (Coleoptera: Curculionidae)
Salvador, Ricardo; Perez Principi, Ricardo Dario; Berretta, Marcelo Facundo; Fernandez, Paula Del Carmen; Paniego, Norma Beatriz; Sciocco, Alicia Ines; Hopp, Horacio Esteban (Entomological Society of America, 2014-11)Anthonomus grandis Boheman is a key pest in cotton crops in the New World. Its larval stage develops within the flower bud using it as food and as protection against its predators. This behavior limits the effectiveness ...