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Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia
Garcia, Araceli Nora; Ayub, Nicolás Daniel; Fox, Ana Romina; Gomez, Maria Cristina; Dieguez, Maria Jose; Pagano, Elba Maria; Berini, Carolina Andrea; Muschietti, Jorge Prometeo; Soto, Gabriela Cinthia (BMC Plant Biology, 2014-09-17)Background: The production of antimicrobial peptides is a common defense strategy of living cells against a wide range of pathogens. Plant snakin peptides inhibit bacterial and fungal growth at extremely low concentrations. ... -
Avances en la generación de mutantes inducidas en especies forrajeras
Garcia, Araceli Nora; Gomez, Maria Cristina; Ladoux, Angeles; Landau, Alejandra Mabel; Prina, Alberto Raul; Pagano, Elba Maria (Sociedad Argentina de Genética, 2018-06)La alfalfa (Medicago sativa L.) y la festuca alta (Festuca arundinacea Schreb.) son dos de las forrajeras templadas más cultivadas en Argentina. Ambas se utilizan en pasturas puras o polifíticas en la región pampeana. La ... -
Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions
Setten, Lorena; Soto, Gabriela Cinthia; Mozzicafreddo, Matteo; Fox, Ana Romina; Lisi, Christian Daniel; Cuccioloni, Massimiliano; Angeletti, Mauro; Pagano, Elba Maria; Diaz Paleo, Antonio Horacio; Ayub, Nicolás Daniel (2013-05-13)Nitrogen is the second most critical factor for crop production after water. In this study, the beneficial rhizobacterium Pseudomonas protegens Pf-5 was genetically modified to fix nitrogen using the genes encoding the ... -
Genetic and morphological characterization of Acroceras macrum Stapf
Ferrari Usandizaga, Silvana Consuelo; Brugnoli, Elsa Andrea; Weiss, Alejandra I.; Zilli, Alex Leonel; Schedler, Mara; Pagano, Elba Maria; Martínez, Eric Javier; Acuña, Carlos Alberto (Wiley, 2015-12)Acroceras macrum is a warm‐season grass used for pastures, and it has high nutritive value. The scarce genetic and reproductive information about this grass is limiting its breeding for forage. The objective of this research ... -
Genetic diversity in a world germplasm collection of tall fescue
Cuyeu, Alba Romina; Rosso, Beatriz Susana; Pagano, Elba Maria; Soto, Gabriela; Fox, Ana Romina; Ayub, Nicolás Daniel (2013)Festuca arundinacea Schreb., commonly known as tall fescue, is a major forage crop in temperate regions. Recently, a molecular analysis of different accessions of a world germplasm collection of tall fescue has demonstrated ... -
The genetic diversity of wild rescuegrass is associated with precipitation levels
Cuyeu, Alba Romina; Pagano, Elba Maria; Rosso, Beatriz Susana; Soto, Gabriela Cinthia; Ayub, Nicolás Daniel (Springer, 2014-04-27)The genus Bromus belongs to the family Poaceae, contains over 160 annual and perennial species of grasses, varying in ploidy level from diploid (2n = 14) to dodecaploid (2n = 70) (Fortune et al. 2008). One of the most ... -
High-quality forage production under salinity by using a salt-tolerant AtNXH1-expressing transgenic alfalfa combined with a natural stress-resistant nitrogen-fixing bacterium
Stritzler, Margarita; Pagano, Elba Maria; Berini, Carolina Andrea; Gomez, Maria Cristina; Ayub, Nicolás Daniel; Soto, Gabriela Cinthia (Elsevier, 2018-06)Alfalfa, usually known as the “Queen of Forages”, is the main source of vegetable protein to meat and milk production systems worldwide. This legume is extremely rich in proteins due to its highly efficient symbiotic ... -
Identificación de cultivares de trébol blanco (trifolium repens l.) mediante SSR
Randazzo, Cecilia; Rosso, Beatriz Susana; Pagano, Elba Maria (Sociedad Argentina de Genética, 2013-07)El trébol blanco (Trifolium repens L) es una leguminosa forrajera de gran valor nutritivo y muy eficiente en la incorporación de nitrógeno (N) al suelo mediante fijación simbiótica. En Argentina se ha difundido ampliamente ... -
Manipulación de la senescencia de alfalfa (Medicago sativa L.) por ingeniería genética
Beltran, Victor Manuel (Facultad de Agronomía, Universidad de Buenos Aires, 2011-12-12)En plantas forrajeras como la alfalfa, la senescencia foliar produce tanto una pérdida de la biomasa de forraje como una reducción de la calidad del mismo. Una estrategia molecular para retrasar la senescencia mediante la ... -
Maximizing the expression of transgenic traits into elite alfalfa germplasm using a supertransgene configuration in heterozygous conditions
Jozefkowicz, Cintia; Frare, Romina Alejandra; Odorizzi, Ariel; Arolfo, Valeria; Pagano, Elba Maria; Basigalup, Daniel Horacio; Ayub, Nicolás Daniel; Soto, Gabriela Cinthia (2018-05)Numerous species of legumes, including alfalfa, are critical factors for agroecosystems due to their ability to grow without nitrogen fertilizers derived from non-renewable fossil fuels, their contribution of organic ... -
Minimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector pPZP200BAR
Bottero, Ana Emilia; Pascuan, Cecilia Gabriela; Pagano, Elba Maria; Ayub, Nicolás Daniel; Soto, Gabriela Cinthia; Jozefkowicz, Cintia (2016)Alfalfa is the most important forage legume worldwide. However, similar to other minor forage crops, it is usually harvested along with weeds, which decrease its nutrient quality and thus reduce its high value in the ... -
Pseudomonas fluorescens Pf-5 genome-wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin-1
Ayub, Nicolás Daniel; Fox, Ana Romina; Garcia, Araceli Nora; Mozzicafreddo, Matteo; Cuccioloni, Massimiliano; Angeletti, Mauro; Pagano, Elba Maria; Soto, Gabriela Cynthia (Oxford University Press, 2015-01)Snakin-1, a peptide produced by higher plants, has broad-spectrum antibiotic activity, inhibiting organisms ranging from Bacteria to Eukaryotes. However, the mode of action against target organisms is poorly understood. ... -
Seasonal Variation of White Clover Morphology at the North of Buenos Aires Province, Argentine
Pagano, Elba Maria; Scheneiter, Jorge Omar (University of Kentucky, 2001)In order to study the seasonal variation in morphological characters that contribute to forage production in white clover (Trifolium repens L.) cultivars, a trial was conducted at Pergamino Agricultural Experimental Station ...