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A closed-tube loop-mediated isothermal amplification assay for the visual endpoint detection of Brucella spp. and Mycobacterium avium subsp. paratuberculosis
Trangoni, Marcos David; Gioffre, Andrea; Cravero, Silvio Lorenzo Pedro (Springer, 2017-06)LAMP (loop-mediated isothermal amplification) is an isothermal nucleic acid amplification technique that is characterized by its efficiency, rapidity, high yield of final product, robustness, sensitivity, and specificity, ... -
Bovine campylobacteriosis in bulls: insights in the conventional and molecular diagnosis
García, Juan Agustín; Farace, Pablo Daniel; Gioffre, Andrea; Morsella, Claudia Graciela; Mendez, Maria Alejandra; Acuña, Joaquín; Aller Atucha, Juan Florencio; Signorini Porchiett, Marcelo Lisandro; Paolicchi, Fernando (Springer Nature, 2023-03)Campylobacter fetus is a gram-negative motile bacterium, with two subspecies relevant to cattle health: C. fetus subsp. venerealis (Cfv) and C. fetus subsp. fetus (Cff). Both subspecies are associated with reproductive ... -
Bovine campylobacteriosis in heifer: pathogenesis study and insights in the conventional and molecular diagnosis in an experimental bovine model and field cases
Garcia, Juan Agustin; Farace, Pablo Daniel; Gioffre, Andrea; Romeo, Florencia; Verna, Andrea Elizabeth; Mendez, Maria Alejandra; Morsella, Claudia Graciela; Aller Atucha, Juan Florencio; Signorini Porchiett, Marcelo Lisandro; Paolicchi, Fernando (Springer Nature, 2024-02)Campylobacter fetus spp. is a bacterium associated to reproductive losses in cattle worldwide. It is a venereal infectious disease known as bovine campilobacteriosis, with high impact mainly in countries with extensive ... -
Campylobacter fetus releases S-layered and immunoreactive outer membrane vesicles = Campylobacter fetus secreta vesículas de membrana externa inmunorreactivas que conservan la capa S
Farace, Pablo; Cravero, Silvio Lorenzo Pedro; Taibo, Catalina Beatriz; Diodati, Julian Angel; Morsella, Claudia Graciela; Paolicchi, Fernando; Sabio Y Garcia, Julia Veronica; Gioffre, Andrea (Elsevier, 2021-08)The study of outer membrane vesicles (OMVs) became relevant because of their probable important role in the transfer of virulence factors to host cells. Campylobacter fetus is mainly a mammal pathogen whose virulence ... -
Characterization of Cellulolytic Activities of Environmental Bacterial Consortia from an Argentinian Native Forest
Romano, Nelson Gaston; Gioffre, Andrea; Sede, Silvana Mabel; Campos, Eleonora; Cataldi, Angel Adrian; Talia, Paola Mónica (Springer, 2013-08)Cellulolytic activities of three bacterial consortia derived from a forest soil sample from Chaco region, Argentina, were characterized. The phylogenetic analysis of consortia revealed two main highly supported groups ... -
Diversidad de especies de micobacterias no tuberculosas aisladas en ambientes acuáticos de la ciudad de General Pico, La Pampa, Argentina = Species diversity of non-tuberculous mycobacteria isolated from aquatic environments of General Pico city, Province of La Pampa (Argentina)
Tortone, Claudia Andrea; Oriani, Delia Susana; Staskevich, Ana Sandra; Oriani, Alejandra Soledad; Gino, Lilia Mabel; Marfil, Maria Jimena; Nava Vargas, Alejandro; Gioffre, Andrea; Zumarraga, Martin Jose (Asociación Argentina de Microbiología, 2018)Las micobacterias no tuberculosas (MNT) no solo se estudian por su importancia como patógenos oportunistas, sino también por sus aplicaciones en biotecnología y biorremediación. Nuestro objetivo fue determinar la presencia ... -
Estudio de la patogenia de Campylobacter fetus en bovinos y utilización de tecnologías moleculares para su detección
García, Juan Agustín (Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, 2022-11)La bacteria Campylobacter fetus, es uno de los principales agentes etiológicos que provocan pérdidas reproductivas en bovinos en Argentina. Hay dos subespecies de C. fetus relevantes para la salud del bovino: C. fetus ... -
Exploring the cellulolytic activity of environmental mycobacteria
Mon, Maria Laura; Romano, Nelson Gaston; Farace, Pablo Daniel; Tortone, Claudia Andrea; Oriani, Delia Susana; Picariello, Gianluca; Zumarraga, Martin Jose; Gioffre, Andrea; Talia, Paola Mónica (Elsevier, 2024-07)Although studies on non-tuberculous mycobacteria have increased in recent years because they cause a considerable proportion of infections, their cellulolytic system is still poorly studied. This study presents a ... -
In vivo short-term exposure to residual oil fly ash impairs pulmonary innate immune response against environmental mycobacterium infection
Delfosse, Veronica Cecilia; Tasat, Deborah Ruth; Gioffre, Andrea (Wiley, 2015-05)Epidemiological studies have shown that pollution derived from industrial and vehicular transportation induces adverse health effects causing broad ambient respiratory diseases. Therefore, air pollution should be taken ... -
Isolation of Campylobacter fetus subsp. venerealis from seminal vesicle of a naturally challenged bull
García, Juan Agustín; Gioffre, Andrea; Acuña, Juaquín; Mendez, Maria Alejandra; Morsella, Claudia Graciela; Aller Atucha, Juan Florencio; Paolicchi, Fernando (Springer Verlag, 2021-09-03)Campylobacter fetus is a well-recognized pathogen that affects reproductive rate in cattle. In the present study, two Angus bulls were kept (39 days) separately with a group of heifers experimentally infected withCampylobacter ... -
L-cysteine transporter-PCR to detect hydrogen sulfide-producing Campylobacter fetus
Farace, Pablo Daniel; Morsella, Claudia Graciela; Cravero, Silvio Lorenzo Pedro; Sioya, Bernardo Arturo; Amadio, Ariel; Paolicchi, Fernando; Gioffre, Andrea (2019-11)Phenotypic differences between Campylobacter fetus fetus and C. fetus venerealis subspecies allow the differential diagnosis of bovine genital campylobacteriosis. The hydrogensulfide production,for example,is atrait exclusive ... -
LAMP technology: Rapid identification of Brucella and Mycobacterium avium subsp. paratuberculosis
Trangoni, Marcos David; Gioffre, Andrea; Ceron Cucchi, Maria Esperanza; Caimi, Karina Cynthia; Ruybal, Paula; Zumarraga, Martin Jose; Cravero, Silvio Lorenzo Pedro (Sociedade Brasileira de Microbiologia, 2015-06)In this study, we developed new sets of primers to detect Brucella spp. and M. avium subsp. paratuberculosis (MAP) through isothermal amplification. We selected a previously well-characterized target gene, bscp31, specific ... -
Mycobacterium intracellulare infection in a capybara (Hydrochoerus hydrochaeris)
Pezzone, Natalia; Eberhardt, Ayelén; Fernandez, Analía; Garbaccio, Sergio Gabriel; Zumarraga, Martin Jose; Gioffre, Andrea; Magni, Carolina; Beldomenico, Pablo Martín; Marini, M. Rocío; Canal, Ana María (American Association of Zoo Veterinarians, 2013-12)This report describes the first case of Mycobacterium intracellulare infection with typical granulomatous lesions of mycobacteriosis in a capybara (Hydrochoerus hydrochaeris). The individual was a captive-bred young female, ... -
Tuberculosis in swine co-infected with Mycobacterium avium subsp. hominissuis and Mycobacterium bovis in a cluster from Argentina
Barandiaran, Soledad; Pérez, Andrés M.; Gioffre, Andrea; Martinez Vivot, Marcela; Cataldi, Angel Adrian; Zumarraga, Martin Jose (Cambridge University Press, 2015-04)In Argentina little is known about the epidemiology of tuberculosis (TB) infection in swine. We characterized the epidemiological dynamics of Mycobacterium avium complex (MAC) infection in a swine population of Argentina ... -
Utilization of molecular and conventional methods for the identification of nontuberculous mycobacteria isolated from different water sources
Tortone, Claudia Andrea; Zumarraga, Martin Jose; Gioffre, Andrea; Oriani, Delia Susana (Wolters Kluwer - Medknow, 2018-03)Background: The environment is the nontuberculous mycobacteria (NTM) reservoir, opportunistic pathogens of great diversity and ubiquity, which is observed in the constant description of new species capable of causing ... -
Virulence factors of the Mycobacterium tuberculosis complex
Forrellad, Marina Andrea; Klepp, Laura Ines; Gioffre, Andrea; Sabio y Garcia, Julia Veronica; Morbidoni, Hector R.; Santangelo, María De La Paz; Cataldi, Angel Adrian; Bigi, Fabiana (Taylor & Francis, 2013-01)The Mycobacterium tuberculosis complex (MTBC) consists of closely related species that cause tuberculosis in both humans and animals. This illness, still today, remains to be one of the leading causes of morbidity and ...