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A direct high-throughput in Cell-ELISA for measuring infectivity of cytopathic and non-cytopathic bovine viral diarrhoea virus strains applied to the assessment of antiviral activity
Quintana, Maria Eugenia; Barone, Lucas; Forlenza, María Belén; Trotta, Myrian Vanesa; Turco, Cecilia; Mansilla, Florencia Celeste; Cardoso, Nancy Patricia; Capozzo, Alejandra Victoria (Elsevier, 2018-07-19)Low-cost high-throughput methods applicable to any virus strain are required for screening antiviral compounds against multiple field strains. Colorimetric cell-viability assays are used for this purpose as long as the ... -
Accuracy of traditional and novel serology tests for predicting cross-protection in foot-and-mouth disease vaccinated cattle
Brito, Barbara Patricia; Perez, Andrés Maximiliano; Capozzo, Alejandra Victoria (Elsevier, 2014-01-16)Foot-and-mouth disease virus (FMDV) antigenic-match between vaccine and field viruses has traditionally been estimated in vitro by computing the r1 value using virus neutralization test (VNT) or ELISA titers. In this study ... -
Actividad del interferón de tipo III contra el virus de la diarrea viral bovina
Quintana, Maria Eugenia (Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, 2019)El Virus de la Diarrea Viral Bovina (VDVB) representa un importante desafío a nivel productivo debido a su carácter endémico a nivel mundial. La infección por el VDVB es altamente prevalente en la Argentina y provoca ... -
Alternatives for the serological assessment of foot-and-mouth disease vaccine immunity in buffaloes (Bubalus bubalis)
Sala, Juan Manuel; Trotta, Myrian Vanesa; Mansilla, Florencia Celeste; Perez Filgueira, Daniel Mariano; Caspe, Sergio Gaston; Capozzo, Alejandra (2018)Buffaloes are compulsory vaccinated against foot-and-mouth disease virus (FMDV) in many countries as part of the official control programmes. Serological testing aimed to indirectly assess herd immunity is currently performed ... -
Apicomplexan profilins in vaccine development applied to bovine neosporosis
Mansilla, Florencia Celeste; Capozzo, Alejandra (2017-12)Neospora caninum, an intracellular protozoan parasite from the phylum Apicomplexa, is the etiologic agent of neosporosis, a disease considered as a major cause of reproductive loss in cattle and neuromuscular disease in ... -
Application of serological tests to assess the efficacy of foot-and-mouth disease vaccination in dairy cattle with or without viral leucosis
Capozzo, Alejandra Victoria (Juniper Publishers, 2018-12)Enzootic bovine leucosis is an infectious viral disease of cattle distributed worldwide that affect dairy cattle over 2 years of age. This disease produces changes in the animal´s immune system that may affect vaccine ... -
Assessment on different vaccine formulation parameters in the protection against heterologous challenge with FMDV in cattle
Di Giacomo, Sebastián; Bucafusco, Danilo; Schammas, Juan Manuel; Pega, Juan Franco; Miraglia, Maria Cruz; Barrionuevo, Florencia Mabel; Capozzo, Alejandra; Perez Filgueira, Daniel Mariano (MDPI, 2022-08)Foot-and-mouth disease (FMD) remains one of the major threats to animal health worldwide. Its causative agent, the FMD virus (FMDV), affects cloven-hoofed animals, including farm animals and wildlife species, inflicting ... -
Bovine interferon lambda Is a potent antiviral against SARS-CoV-2 infection in vitro
Cardoso, Nancy; Mansilla, Florencia Celeste; Benedetti, Estefanía; Turco, Cecilia Soledad; Barone, Lucas Jose; Iserte, Javier Alonso; Soria, Ivana; Baumeister, Elsa; Capozzo, Alejandra (Frontiers Media, 2020-11)Interferon lambda (IFN-λ) is an antiviral naturally produced in response to viral infections, with activity on cells of epithelial origin and located in the mucosal surfaces. This localized activity results in reduced ... -
Chronic NOD2 stimulation by MDP confers protection against parthanatos through M2b macrophage polarization in RAW264.7 cells
Mansilla, Florencia Celeste; Miraglia, Maria Cruz; Maidana, Silvina Soledad; Randazzo, Cecilia Paola; Capozzo, Alejandra (Elsevier, 2024-09)Innate immune cells show enhanced responsiveness to secondary challenges after an initial non-related stimulation (Trained Innate Immunity, TII). Acute NOD2 activation by Muramyl-Dipeptide (MDP) promotes TII inducing the ... -
Development and validation of a novel ELISA for the detection of Neospora caninum antibodies in bovine sera
Mansilla, Florencia Celeste; Gual, Ignacio; Pereyra, Walter Rodrigo; Cardoso, Nancy Patricia; Turco, Cecilia; Capozzo, Alejandra Victoria (Annex Publishers, 2019-08-26)Neospora caninum is a world-wide distributed apicomplexan parasite, causative agent of bovine neosporosis, which is one of the major causes of reproductive losses in cattle affecting both dairy and beef industries. Several ... -
Dying alone due to COVID-19 : do the needs of the many outweigh the rights of the few—or the one?
Capozzo, Alejandra (Frontiers Media, 2020-11)The COVID-19 pandemic, caused by infection with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has been striking the world since it was first identified in December 2019 in China. The World Health ... -
Early Adaptive Immune Responses in the Respiratory Tract of Foot-and-Mouth Disease Virus-Infected Cattle
Pega, Juan Franco; Bucafusco, Danilo; Di Giacomo, Sebastián; Schammas, Juan Manuel; Malacari, Darío Amilcar; Capozzo, Alejandra Victoria; Arzt, J.; Pérez-Beascoechea, C.; Maradei, E.; Rodríguez, L.L.; Borca, Manuel Victor; Perez Filgueira, Daniel Mariano (2013-03)Foot-and-mouth disease (FMD) is a highly contagious viral disease which affects both domestic and wild biungulate species. This acute disease, caused by the FMD virus (FMDV), usually includes an active replication phase ... -
Editorial : Foot-and-mouth disease epidemiology, vaccines and vaccination : moving forward
Capozzo, Alejandra; Vosloo, Wilna; de los Santos, Teresa; Pérez, Andrés M.; Perez Filgueira, Daniel Mariano (Frontiers Media, 2023-06)Vaccination has played a major role in foot-and-mouth disease (FMD) control. There are different approaches to the design and implementation of vaccination campaigns, and epidemiological information is paramount in influencing ... -
Editorial : Women in veterinary epidemiology and economics
Capozzo, Alejandra; Vial, Flavie (Frontiers Media, 2023-05)While the number of women graduating from veterinary schools has increased globally over the last few decades, this has not translated into reduced gender bias and inequity in academia and veterinary science research (1). ... -
Estimating the protection afforded by foot-and-mouth disease vaccines in the laboratory
Paton, D. J.; Reeve, R.; Capozzo, Alejandra Victoria; Ludi, A. (Elsevier, 2019-09)Foot-and-mouth disease (FMD) vaccines must be carefully selected and their application closely monitored to optimise their effectiveness. This review covers serological techniques for FMD vaccine quality control, including ... -
Evaluation of immune responses of stabilised SAT2 antigens of foot-and-mouth disease in cattle
Scott, Katherine A.; Rathogwa, N.M.; Capozzo, Alejandra; Maree, Francois F. (2017)Foot-and-mouth disease (FMD) vaccines with improved stability and less reliant on a cold-chain are needed to improve the longevity of immune responses elicited in animals. This is especially so for serotypes O and SAT2 ... -
Evaluation of serological response to foot-and-mouth disease vaccination in BLV infected cows
Puentes, Rodrigo; De Brun, Laureana; Algorta, Agustina; Da Silva, Valeria; Mansilla, Florencia Celeste; Sacco, Gustavo; Llambí, Silvia; Capozzo, Alejandra Victoria (2016)Bovine Leukemia Virus (BLV) produces disorders on the immune system in naturally infected animals, which may counteract the development of immunity after vaccination. The aim of this study was to investigate whether healthy ... -
Evidence of reduced vertical transmission of Neospora caninum associated with higher IgG1 than IgG2 serum levels and presence of IFN-γ in nonaborting chronically infected cattle under natural condition
Pereyra, Walter Rodrigo; Mansilla, Florencia Celeste; Petersen, Marcos Iván; Suarez, Victor Humberto; Capozzo, Alejandra Victoria (Elsevier, 2019-02)Neospora caninum infection of cattle can be vertically transmitted, resulting in abortion or birth of infected calves. Vertical transmission occurs both in acutely or chronically infected cattle. There is little information ... -
Fasciola hepatica infection modifies IgG1 specific immune response to foot-and-mouth disease virus induced by vaccination
Costa, Monique; Mansilla, Florencia Celeste; Sala, Juan Manuel; Saravia, Anderson; Ubios, Diego; Lores, Pablo; Capozzo, Alejandra; Freire, Teresa (Elsevier, 2024-01)Fasciola hepatica, a worldwide distributed helminth, has a robust immunoregulatory effect in the host, increasing the susceptibility to secondary infections. Foot and mouth disease (FMD) is a highly contagious acute vesicular ... -
Foot-and-mouth disease vaccination induces cross-reactive IFN-γ responses in cattle that are dependent on the integrity of the 140S particles
Bucafusco, Danilo; Di Giacomo, Sebastián; Pega, Juan Franco; Schammas, Juan Manuel; Cardoso, Nancy Patricia; Capozzo, Alejandra Victoria; Perez Filgueira, Daniel Mariano (2015-02)Interferon-γ (IFN-γ) recall responses against foot-and-mouth disease virus (FMDV) in FMD vaccinated cattle are utilized to study T-lymphocyte immunity against this virus. Here, a recall IFN-γ assay based on a commercial ...