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A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs
Obiegala, Anna; Król, Nina; Heyse, Lara M.I.; Pfeffer, Martin; Montini, Martina; Nava, Santiago; Sebastian, Patrick (Springer, 2025-05)The aim of the study was to establish an artificial feeding system (AFS), based on silicon membranes, for Amblyomma sculptum nymphs and compare it to classical feeding systems using laboratory animals. Three cohorts of 60 ... -
Analysis of the tick communities associated to domestic mammals in rural areas of the Yungas montane forest from Argentina
Copa, Griselda Noemi; Flores, Fernando Sebastián; Tarragona, Evelina Luisa; Lamattina, Daniela; Sebastian, Patrick; Gil, José F.; Mangold, Atilio Jose; Venzal, José Manuel; Nava, Santiago (Elsevier, 2023-04)The aim of this work was to describe the tick community associated to domestic mammals in rural areas from the Yungas lower montane forest of Argentina. The circulation of tick-borne pathogens was also analyzed. Samples ... -
Assays to evaluate the transovarial transmission of Anaplasma marginale by Rhipicephalus microplus
Mazzucco Panizza, Matilde Nahimé; Cutulle, Christian; Primo, María Evangelina; Morel, Nicolas; Sebastian, Patrick; Nava, Santiago (Elsevier, 2022-11)The aim of this study was to evaluate the vectorial competence of Rhipicephalus microplus to transmit Anaplasma marginale transovarially, by analyzing the results of three different but complementary assays. First, larvae ... -
Babesia sp. of the “South American Marsupialia Group”: First molecular detection in Didelphis albiventris (Didelphimorphia: Didelphidae) from Argentina
Sebastian, Patrick; Lamattina, Daniela; Vaschalde, Paula Josefina; Flores, Fernando Sebastián; Torrents, Jorgelina; Tarragona, Evelina Luisa (Elsevier, 2025-02)Field captured specimens of Didelphis albiventris (Didelphimorphia: Didelphidae) from Formosa Province, Argentina, were analysed for possible infection with Babesia species. Whole blood samples of live-trapped D. albiventris ... -
Bacteria of the genera Ehrlichia and Rickettsia in ticks of the family Ixodidae with medical importance in Argentina
Tarragona, Evelina Luisa; Saracho Bottero, María Noelia; Mangold, Atilio Jose; Sebastian, Patrick Stephan; Mackenstedt, Ute; Nava, Santiago (2016)The aim of this study was to get an overview about the occurrence of bacteria from the genus Ehrlichia and Rickettsia in ixodid ticks with medical importance in Argentina. Therefore, in 2013 and 2014, free-living ticks ... -
Borrelia genospecies in Ixodes sp. cf. Ixodes affinis (Acari: Ixodidae) from Argentina
Flores, Fernando Sebastián; Saracho Bottero, Maria Noelia; Sebastian, Patrick Stephan; Venzal, José Manuel; Mangold, Atilio Jose; Nava, Santiago (Elsevier, 2020-11)The aim of this work was to evaluate the presence of Borrelia infection in Ixodes sp. cf. Ixodes affinis ticks from Argentina. Specimens of Ixodes sp. cf. I. affinis were collected on vegetation and birds in five locations ... -
Development and evaluation of a TaqMan® real-time PCR assay for species-specific detection of Ehrlichia canis
Sarli, Macarena; De Salvo, María N.; Díaz Pérez, Paula M.; Cicuttin, Gabriel L.; Nava, Santiago; Sebastian, Patrick (Elsevier, 2024-12)The aim of this work was to develop a real-time PCR assay with a TaqMan® probe that detects a species-specific part of the 16S rDNA gene of Ehrlichia canis. Canine blood samples (n = 207), collected and tested by a ... -
Epidemiological link between canine monocytic ehrlichiosis caused by Ehrlichia canis and the presence of Rhipicephalus sanguineus sensu stricto in Argentina
Sebastian, Patrick Stephan; Mera y Sierra, Roberto; Neira, Gisela; Hadid, Jaled; Flores, Fernando Sebastian; Nava, Santiago (Springer, 2021-01)In this work, we analyze data that support an epidemiological link between cases of canine monocytic ehrlichiosis (CME) by Ehrlichia canis and the presence of Rhipicephalus sanguineus sensu stricto as vector in an endemic ... -
Evaluation of the transmission of Anaplasma marginale by Amblyomma tonelliae
Mazzucco Panizza, Matilde Nahimé; Tarragona, Evelina Luisa; Sebastian, Patrick; Flores, Fernando Sebastian; Mangold, Atilio Jose; Nava, Santiago (Elsevier, 2025-02)This study aimed to evaluate A. marginale transstadial (TST) and transovarial transmission (TOT) by Amblyomma tonelliae through vector competence assays and analysis of natural infection in free-living ticks. This three-host ... -
Exotic tick detected in Argentina on a tourist returning from South Africa
Tarragona, Evelina Luisa; Sebastian, Patrick Stephan; Guglielmone, Alberto; Nava, Santiago (Elsevier, 2021-02-12)The aim of this study was to report the finding of a nymph attached to an Argentinean tourist returning from South Africa. The nymph specimen was morphologically analysed, submitted to DNA extraction and amplifying the 16S ... -
First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, Argentina
Sebastian, Patrick; Vaschalde, Paula Josefina; Pelosi, María Celeste; Flores, Fernando Sebastián; Yedro, Julio; Tarragona, Evelina Luisa (MDPI, 2025-09)In November 2022, a male specimen of Subulo gouazoubira was found with signs of obnubilation and recumbency without the ability to maintain sternal or ambulatory posture. Based on the symptoms, serological, biochemical and ... -
Ixodes chilensis Kohls, 1956 (Acari: Ixodida: Ixodidae) in Argentina
Cicuttin, Gabriel L.; Sebastian, Patrick; Lombardo, Maria Alejandra; Simone, Marcela; De Salvo, Maria Nazarena; Nava, Santiago (Elsevier, 2025-10)The aim of this note is to report the first record of Ixodes chilensis Kohl, 1956 in Argentina. In June 2023, ticks were collected on bat belonging to the genus Histiotus from Dina Huapí, Rio Negro Province, Patagonian ... -
Molecular detection and phylogenetic analysis of Anaplasma platys-like and Candidatus Anaplasma boleense strains from Argentina
Sebastian, Patrick; Mazzucco Panizza, Matilde Nahimé; García Ríos, Ignacio José María; Tarragona, Evelina Luisa; Trova, Gabriela Beatriz; Sánchez Negrette, Olga; Primo, María Evangelina; Nava, Santiago (Elsevier, 2023-05)The present study aimed at the molecular detection of Anaplasma spp. in different samples obtained from cattle, goats and free-living Rhipicephalus microplus ticks from Argentina. DNA of members of the Anaplasmataceae ... -
Molecular detection and phylogenetic characterization of Theileria equi in horses (Equus caballus) from a peri-urban area of Argentina
Sebastian, Patrick Stephan; Benitez Ibalo, Alicia P.; Flores, Fernando Sebastian; Debárbora, Valeria Natalia; Martinez, Emilia Irina; Thompson, Carolina Soledad; Mangold, Atilio José (Elsevier, 2021-11)To investigate the presence of Theileria equi in an endemic area of equine piroplasmosis 42 horses (Equus caballus) from Corrientes City, Argentina were sampled. Eighty-one percent (34 blood samples) of the analyzed horses ... -
Molecular detection of Candidatus Ehrlichia pampeana (Rickettsiales: Anaplasmataceae) in Haemaphysalis juxtakochi (Acari: Ixodidae) from central Argentina
Flores, Fernando Sebastian; Sebastian, Patrick Stephan; Nava, Santiago (Springer, 2024-02)The aim of this study was to report the first detection of Candidatus Ehrlichia pampeana in Haemaphysalis juxtakochi from Argentina. Free-living ticks were collected from vegetation by drag-flag method on five sample sites ... -
Molecular Detection of Candidatus Rickettsia andeanae and Ehrlichia sp. in Amblyomma pseudoconcolor Aragão, 1908 (Acari: Ixodidae) from the Argentinian Patagonia
Sebastian, Patrick; Winter, Marina; Abate, Sergio Damián; Tarragona, Evelina Luisa; Nava, Santiago (MDPI, 2022-11)This study presents the molecular detection of Candidatus Rickettsia andeanae and Ehrlichia sp. in Amblyomma pseudoconcolor Aragão, 1908 (Acari: Ixodidae) collected on a large hairy armadillo (Chaetophractus villosus ... -
Molecular detection of rickettsial bacteria in ticks of the genus Ixodes from the Southern Cone of America
Sebastian, Patrick Stephan; Flores, Fernando Sebastian; Saracho Bottero, Maria Noelia; Tarragona, Evelina Luisa; Venzal, José Manuel; Nava, Santiago (Elsevier, 2020-10)To obtain information about rickettsial bacteria in ticks of the genus Ixodes from Argentina and Uruguay, specimens of I. fuscipes (previously named as I. aragaoi), Ixodes pararicinus, Ixodes sp. cf. I. affinis, and Ixodes ... -
Molecular genotyping of Theileria spp. detected in horses from Corrientes City, Argentina
Benitez Ibalo, Alicia P.; Debárbora, Valeria Natalia; Mangold, Atilio Jose; Nava, Santiago; Sebastian, Patrick (Springer, 2024-12-24)After the previous description and characterization of Theileria equi sensu stricto (Genotype A) in Argentina, the aim of the present study was to investigate the possible heterogeneity of 18 S rDNA genotypes circulating ... -
Molecular genotyping of Theileria spp. detected in horses from Corrientes City, Argentina
Benitez Ibalo, Alicia P.; Debárbora, Valeria Natalia; Mangold, Atilio Jose; Nava, Santiago; Sebastian, Patrick (Springer, 2025-02)After the previous description and characterization of Theileria equi sensu stricto (Genotype A) in Argentina, the aim of the present study was to investigate the possible heterogeneity of 18 S rDNA genotypes circulating ... -
Novel Anaplasma (Rickettsiales: Anaplasmataceae) strain and Hepatozoon sp. cf. H. procyonis (Apicomplexa, Hepatozoidae) detected in Procyon cancrivorus (Carnivora, Procyonidae) from Argentina, with note of tick-host association
Tarragona, Evelina Luisa; Sebastian, Patrick; Félix, Maria L.; Venzal, José Manuel (Springer, 2023-04)The aim of this work is to report the first detection of Procyon cancrivorus naturally co-infected with Hepatozoon sp. cf. H. procyonis and a novel Anaplasma strain from South America and potential vector tick species ...