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Aplicación de un método de extracción de ADN a partir de sangre canina sensible y de bajo costo para el diagnóstico molecular de Leishmania sp. = Application of a sensitive and low cost method for the extraction of DNA from canine blood to the molecular diagnosis of Leishmania sp.
Ascencio, Mariano; Florin-Christensen, Mónica; Schnittger, Leonhard; Rodriguez, Anabel Elisa (Universidad de Morón, 2017-10)La leishmaniosis canina (LC) es una enfermedad causada por hemoparásitos del género Leishmania. Los parásitos se transmiten a través de vectores flebótomos a los seres humanos y a otros mamíferos, generando cuadros ... -
Cysteine proteinase C1A paralog profiles correspond with phylogenetic lineages of pathogenic piroplasmids
Ascencio, Mariano; Florin-Christensen, Mónica; Mamoun, Choukri B.; Weir, William; Shiels, Brian; Schnittger, Leonhard (MDPI, 2018-04)Piroplasmid parasites comprising of Babesia, Theileria, and Cytauxzoon are transmitted by ticks to farm and pet animals and have a significant impact on livestock industries and animal health in tropical and subtropical ... -
Kinetic characterization of a novel cysteine peptidase from the protozoan Babesia bovis, a potential target for drug design
Lu, Stephen; Ascencio, Mariano; Torquato, Ricardo J.S.; Florin-Christensen, Mónica; Tanaka, Aparecida S. (Elsevier, 2020-12)C1A cysteine peptidases have been shown to play an important role during apicomplexan invasion and egress of host red blood cells (RBCs) and therefore have been exploited as targets for drug development, in which peptidase ... -
Molecular diagnosis of Leishmania spp. in dogs of a subtropical locality of Argentina
Ascencio, Mariano; Sarmiento, Nestor Fabian; Schnittger, Leonhard; Florin-Christensen, Mónica; Rodriguez, Anabel Elisa (Wiley, 2020-07)Leishmaniosis is a tropical and subtropical vector‐borne disease caused by hemoparasites of the genus Leishmania. The disease can infect humans, as well as domestic and wildlife animals. Dogs are the main reservoir for L. ... -
Molecular evidence of Leishmania spp. in spider monkeys (Ateles geoffroyi) from The Tuxtlas Biosphere Reserve, Veracruz, Mexico
Pérez-Brígido, Carlos D.; Romero-Salas, Dora; Pardío-Sedas, Violeta T.; Cruz-Romero, Anabel; González-Hernández, Milagros; Delprá-Cachulo, Joyce Mara; Ascencio, Mariano; Florin-Christensen, Mónica; Schnittger, Leonhard; Rodriguez, Anabel Elisa (Springer, 2021-10)The black-handed spider monkey (Ateles geoffroyi) is a platyrrhine primate distributed in southern Mexico, Central America, and part of South America. Two subspecies inhabit Mexico: Ateles geoffroyi vellerosus and Ateles ... -
Use of Chelex-100 for the molecular diagnosisof five animal pathogens
Tomazic, Mariela Luján; Hamer, Micaela; Bustos, Carla Paola; Arregui, Matias Ezequiel; Ascencio, Mariano; Saraullo, Vanina Rosa; Watanabe, Olivia; Brihuega, Bibiana Felicitas; Rodriguez, Anabel Elisa; Grune Loffler, Sylvia (Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, 2021-06)Molecular tools have improved conventional veterinary diagnosis. Acid nucleic extraction is a key step for downstream applications. This work aimed to compare the DNA extraction method Chelex-100 resin (M1) ...