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A simple and economic three-step process for producing highly purified Fab’ fragments directly from the egg yolk water-soluble fraction
González Viacava, Myriam Belén; Leiva, Carlos Leónidas; Tavarone, Maria Eugenia; Dokmetjian, José Christian; Cascone, Osvaldo; Chacana, Pablo; Fingermann, Matías (Elsevier, 2022-11)Although mammals are still the main source of polyclonal antibodies production, the use of egg yolk immunoglobulins (IgY) is increasing, and thus improvement of its safety to reduce undesired reactions is needed. Removal ... -
Desarrollo de alternativas diagnósticas de tuberculosis bovina
Leiva, Carlos (Universidad Nacional de Quilmes, 2015-02)En el siguiente trabajo se llevó cabo la puesta a punto y optimización de la técnica LAMP (del inglés loop-mediated isothermal amplification) para la detección de Mycobacterium bovis, agente etiológico de la tuberculosis ... -
Evaluación de un ensayo de amplificación isotérmica mediada por bucles (LAMP) para detectar el gen stx2 en aislamientos clínicos de Escherichia coli en Argentina
Leiva, Carlos; Rivero, Rocío; Redondo, Leandro Martín; Fernandez Miyakawa, Mariano Enrique; Bruno, Susana B.; Rogé, Ariel Diego (2015-09)Escherichia coli productor de toxina Shiga (STEC del inglés Shiga toxin-producing Escherichia coli) es un patógeno de transmisión alimentaria que puede producir enfermedades en humanos tales como diarreas hemorrágicas así ... -
IgY technology: producing antibodies from a 3Rs perspective
Leiva, Carlos Leónidas; Casanova, Natalia Andrea; Fernandez Miyakawa, Mariano Enrique; Chacana, Pablo (2018-07) -
IgY-technology (egg yolk antibodies) in human medicine: a review of patents and clinical trials
Leiva, Carlos; Gallardo, Mauro Julián; Casanova, Natalia Andrea; Terzolo, Horacio; Chacana, Pablo (Elsevier, 2020-04)IgY-technology (the production and extraction of specific IgY antibodies from egg yolk) is an innovative method to produce antibodies for therapy and prophylaxis. Advantages of IgY over other antibodies comprise its ... -
In vitro inhibitory activity of IgY antibodies against Salmonella ser. newport isolated from horses
Bustos, Carla Paola; Leiva, Carlos Leónidas; Gambarotta, Mariana; Guida, Nora; Chacana, Pablo (Elsevier, 2021-08)Equine salmonellosis is caused by several Salmonella serotypes, including Salmonella Newport, which cause enterocolitis and diarrhea. Treatment usually includes the administration of antibiotics. However, since multidrug-resistant ... -
In vivo neutralization of bee venom lethality by IgY antibodies
Leiva, Carlos Leónidas; Geoghegan, Patricia; Lammer, Mónica; Cangelosi, Adriana; Mariconda, Virginia; Celi Castillo, Ana Beatriz; Brero, Luisa; Chacana, Pablo (Elsevier, 2021-07)Bee venom is a complex mixture of molecules, among which melittin and phospholipase A2 (PLA2) are the toxic components involved in envenoming accidents with multiple honeybee stings. Traditionally, the treatment of envenomings ... -
Use of adjuvant ISA VG 71 to produce neutralizing egg yolk antibodies against bothropic venom
Leiva, Carlos Leónidas; Cangelosi, Adriana; Mariconda, Virginia; Celi Castillo, Ana Beatriz; Joaquim, Patricia Estefania; Geoghegan, Patricia; Fernandez Miyakawa, Mariano Enrique; Chacana, Pablo (Springer, 2023-02)The use of egg yolk antibodies—IgY technology—represents an alternative to the production of mammalian immunoglobulins and has several advantages regarding animal welfare and lower costs of production. The use of adjuvants ...