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Arabidopsis DNA glycosylase MBD4L improves recovery of aged seeds
Lescano Lopez, Carlos Ignacio; Torres, José Roberto; Cecchini, Nicolás Miguel; Alvarez, María Elena (Wiley, 2024-07-04)DNA glycosylases initiate the base excision repair (BER) pathway by catalyzing the removal of damaged or mismatched bases from DNA. The Arabidopsis DNA glycosylase methyl-CpG-binding domain protein 4 like (MBD4L) is a ... -
A comet assay protocol for analysis of DNA damage in cryopreserved nuclei from Arabidopsis seeds, seedlings, and leaves
Torres, José Roberto; Lescano Lopez, Carlos Ignacio; Alvarez, María Elena (Elsevier, 2025-12)The comet assay is classically applied to study DNA damage at the single-cell level. In plants, it is commonly used for the analysis of seedlings, leaves, and seeds. The assay is time-consuming and runs continuously, making ... -
The Arabidopsis chromatin regulator MOM1 is a negative component of the defense priming induced by AZA, BABA and PIP
Miranda de la Torre, Julián O.; Peppino Margutti, Micaela Y.; Lescano Lopez, Carlos Ignacio; Cambiagno, Damian Alejandro; Alvarez, María E.; Cecchini, Nicolás M. (Frontiers Media, 2023-05-09)In plants, the establishment of broad and long-lasting immunity is based on programs that control systemic resistance and immunological memory or “priming”. Despite not showing activated defenses, a primed plant induces a ... -
Turnip Mosaic Virus impairs thermomorphogenetic responses in Arabidopsis plants amid genetic and hormonal disruption
Lescano Lopez, Carlos Ignacio; Yañez Santos, Anahí Mara; Realini, Maria Florencia; Lascano, Hernan Ramiro; Asurmendi, Sebastian; Manacorda, Carlos Augusto (Sociedad Argentina de Fisiología Vegetal, 2025-08)BACKGROUND: • Plants rely on thermomorphogenesis (TMG) to adapt their growth and development in response to moderate temperature increases, a process mainly regulated by the PIF4 (Phytochrome Interacting Factor 4) transcription ...