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Evaluation of PMA‐qPCR methodology to detect and quantify viable Shiga toxin‐producing Escherichia coli in beef burgers
Rey, Maria De Los Angeles; Cap, Mariana; Favre, Leonardo Cristian; Rodriguez, Anabel; Dus Santos, Maria Jose; Vaudagna, Sergio Ramon; Mozgovoj, Marina Valeria (2021-02-06)Propidium monoazide (PMA) is a selective nucleic acid intercalating dye that can be combined with real‐time PCR (qPCR) in order to evaluate cell viability in food samples. The aim of the present work was to evaluate PMA‐qPCR ... -
Optimising the Polyphenolic Content and Antioxidant Activity of Green Rooibos (Aspalathus linearis) Using Beta-Cyclodextrin Assisted Extraction
Vhangani, Lusani Norah; Favre, Leonardo Cristian; Rolandelli, Guido; Van Wyk, Jessy; Buera, María del Pilar (2022-05-27)Antioxidant activity associated with green rooibos infusions is attributed to the activity of polyphenols, particularly aspalathin and nothofagin. This study aimed to optimise β-cyclodextrin (β-CD)-assisted extraction of ... -
The complex dependence of non-enzymatic browning development on processing conditions in maize snacks
Rolandelli, Guido; Favre, Leonardo Cristian; Mshicileli, Ndumiso; Vhangani, Lusani Norah; Farroni, Abel Eduardo; Van Wyk, Jessy; Buera, María del Pilar (Elsevier, 2021-04-30)The complex dependence of non-enzymatic browning development on processing conditions was analyzed in the production of maize snacks. The influence of the amount of water added to maize flour, toasting time, and toasting ... -
Use of propidium monoazide coupled with real-time PCR methodology to evaluate Escherichia coli O157 lethality in beef burgers after organic acid addition
Rey, María De Los Ángeles; Cap, Mariana; Favre, Leonardo Cristian; Vaudagna, Sergio Ramon; Mozgovoj, Marina Valeria (Institute of Food Technologists (IFT), 2025-03)In order to achieve bacterial inactivation in beef burgers, one of the alternatives proposed is to add organic acids as part of their formulation. The aim of this work was to evaluate the effectiveness of propidium monoazide ...