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Development and field evaluation of a competitive ELISA to detect specific antibodies against Anaplasma marginale
Bellezze, Julio; Valentini, Beatriz Susana; Bosio, Anabela; Thompson, Carolina Soledad; Primo, María Evangelina (Elsevier, 2026-02)A species-specific competitive ELISA for Anaplasma marginale antibodies detection (cELISA-sAm) was developed by using both a monoclonal antibody specific to an epitope of MSP5 of A. marginale (Am6-mAb) and adding a ... -
Development and field evaluation of a nested polymerase chain reaction-restriction fragment length polymorphism (nPCR-RFLP) analysis to identify A. marginale-infected and A. centrale-vaccinated cattle
Primo, María Evangelina; Bellezze, Julio; Morel, Nicolas; Mazzucco Panizza, Matilde Nahimé; Valentini, Beatriz Susana; Torioni, Susana Marta; Thompson, Carolina Soledad (Elsevier, 2022-07)A nested polymerase chain reaction-restriction fragment length polymorphism (nPCR-RFLP) assay based on the amplification of the Anaplasma spp. highly conserved msp5 gene and posterior digestion with HindIII endonuclease ... -
Development and field evaluation of an ELISA to differentiate Anaplasma marginale–infected from A. centrale–vaccinated cattle
Bellezze, Julio; Thompson, Carolina Soledad; Bosio, Anabela; Torioni, Susana Marta; Primo, María Evangelina (American Association of Veterinary Laboratory Diagnosticians, 2023-03)Immunization of calves with Anaplasma centrale is used to prevent acute anaplasmosis caused by A. marginale. Natural and vaccine-acquired immunity is detected through serologic tests based primarily on A. marginale recombinant ...