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Development and field evaluation of a nested polymerase chain reaction-restriction fragment length polymorphism (nPCR-RFLP) analysis to identify A. marginale-infected and A. centrale-vaccinated cattle

Primo, María Evangelina; Bellezze, Julio; Morel, Nicolas; Mazzucco Panizza, Matilde Nahimé; Valentini, Beatriz Susana; Torioni, Susana Marta; Thompson, Carolina Soledad (Elsevier, 2022-07)

A nested polymerase chain reaction-restriction fragment length polymorphism (nPCR-RFLP) assay based on the amplification of the Anaplasma spp. highly conserved msp5 gene and posterior digestion with HindIII endonuclease ...
Development and field evaluation of an ELISA to differentiate Anaplasma marginale–infected from A. centrale–vaccinated cattle

Bellezze, Julio; Thompson, Carolina Soledad; Bosio, Anabela; Torioni, Susana Marta; Primo, María Evangelina (American Association of Veterinary Laboratory Diagnosticians, 2023-03)

Immunization of calves with Anaplasma centrale is used to prevent acute anaplasmosis caused by A. marginale. Natural and vaccine-acquired immunity is detected through serologic tests based primarily on A. marginale recombinant ...