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Comparative analysis of CRISPR/Cas9 delivery approaches for polyphenol oxidase 2 gene editing in potato
Gonzalez, Matías Nicolás; Massa, Gabriela Alejandra; Anderson, Mariette; Decima Oneto, Cecilia Andrea; Turesson, Helle; Storani, Leonardo; Olsson, Niklas; Fält, Ann Sofie; Hofvander, Per; Feingold, Sergio Enrique (Keystone Symposia on Molecular and Cellular Biology, 2021-03)StPPO2 gene editing was analyzed by Agrobacterium mediated transformation with CR-PPO vector, ribonucleoprotein complexes (RNP-PPO) transfection to protoplasts, and CR-PPO transient expression in protoplasts, yielding ... -
CRISPR/Cas9 for potato functional genomics and breeding
Gonzalez, Matías Nicolás; Massa, Gabriela Alejandra; Andersson, Mariette; Storani, Leonardo; Olsson, Niklas; Decima Oneto, Cecilia Andrea; Hofvander, Per; Feingold, Sergio Enrique (Humana Press (Springer), 2023-03-31)Cultivated potato (Solanum tuberosum L.) is one of the most important staple food crops worldwide. Its tetraploid and highly heterozygous nature pose a great challenge to its basic research and trait improvement through ... -
El diagnóstico de COVID-19 en Balcarce como parte del enfoque “Una Salud”
Louge Uriarte, Enrique Leopoldo; Romeo, Florencia; Rey, Florencia; Canton, German Jose; Storani, Leonardo; Moore, Prando Dadin; Zimmermann, Julián; Verna, Andrea Elizabeth (Estación Experimental Agropecuaria Balcarce, INTA, 2021-11)En el artículo, presentamos el trayecto recorrido por nuestro equipo en respuesta a la necesidad concreta de la sociedad Balcarceña, contar con la posibilidad de diagnosticar COVID19 a nivel local. -
Frequency of bovine viral diarrhea virus (BVDV) in Argentinean bovine herds and comparison of diagnostic tests for BVDV detection in bovine serum samples: a preliminary study
Spetter, Lucas Maximiliano; Louge Uriarte, Enrique Leopoldo; Armendano, Joaquín Ignacio; Alvarez, Ignacio; Norero, Natalia Sigrid; Storani, Leonardo; Pereyra, Susana Beatriz; Verna, Andrea Elizabeth; Odeon, Anselmo Carlos; Gonzalez Altamiranda, Erika (Elsevier, 2020-11)Bovine viral diarrhea (BVD) is a major worldwide disease with negative economic impact on cattle production. Successful control programs of BVD require the identification and culling of persistently infected (PI) animals ... -
Reduced enzymatic browning in potato tubers by specific editing of a Polyphenol oxidase gene via Ribonucleoprotein complexes delivery of the CRISPR/Cas9 System.
Gonzalez, Matías Nicolás; Massa, Gabriela Alejandra; Andersson, Mariette; Turesson, Helle; Olsson, Niklas; Fält, Ann-Sofie; Storani, Leonardo; Decima Oneto, Cecilia Andrea; Hofvander, Per; Feingold, Sergio Enrique (Frontiers Media, Switzerland, 2020-01-09)Polyphenol Oxidases (PPOs) catalyze the conversion of phenolic substrates to quinones, leading to the formation of dark-colored precipitates in fruits and vegetables. This process, known as enzymatic browning, is the cause ... -
Study of the dynamics of in vitro infection with bovine gammaherpesvirus type 4 and apoptosis markers in susceptible cells
Romeo, Florencia; Delgado, Santiago Germán; Louge Uriarte, Enrique Leopoldo; Storani, Leonardo; Martínez Cuesta, Lucia; Morán, Pedro; González Altamiranda, Erika; Odeon, Anselmo Carlos; Pérez, Sandra (Elsevier, 2022-08)Bovine gammaherpesvirus type 4 (BoHV-4) shows tropism for the endometrium, in which it causes the death of epithelial and stroma cells. Despite having anti-apoptotic genes in its genome, experiments based on immortalized ... -
Transformación eficiente de Solanum tuberosum cv. Spunta mediada por Agrobacterium tumefaciens utilizando higromicina como agente selectivo
Decima Oneto, Cecilia Andrea; Coronel, Joel; Storani, Leonardo; Gonzalez, Matías Nicolás; Feingold, Sergio Enrique; Massa, Gabriela Alejandra (Ediciones INTA, 2020-08)La transformación genética de plantas es una herramienta biotecnológica de alto impacto en el mejoramiento vegetal que brinda la posibilidad de obtener nuevos cultivos que se adapten a las demandas de producción y de ... -
Transmisión “intergeneracional” (agámica) de la tolerancia al estrés hídrico en Solanum tuberosum cv. Achat
Storani, Leonardo; Yanovsky, Marcelo J. (Gerencia de Comunicación e Imagen Institucional, DNA SICC, INTA, 2018-12)Los diferentes estreses tanto bióticos como abióticos afectan diversos cultivos y causan grandes pérdidas económicas. En los últimos años se han desarrollado estrategias para obtener plantas tolerantes a los diferentes ...