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Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus
Pujato, Silvina A.; Galliani, Valentina; Irazoqui, Jose Matias; Amadio, Ariel; Quiberoni, Andrea; Mercanti, Diego (Elsevier, 2021-07)A total of 49 strains of Lacticaseibacillus were screened for the presence of CRISPR-Cas systems. Type II-A CRISPR-Cas loci were sequenced for 8 Lacticaseibacillus paracasei and 3 Lacticaseibacillus rhamnosus strains. They ... -
Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis
Kurth, Daniel German; Amadio, Ariel F.; Ordoñez, Omar F.; Allbarracín, Virginia H.; Farías, María E.; Gärtner, Wolfgang (2017)Modern stromatolites thrive only in selected locations in the world. Socompa Lake, located in the Andean plateau at 3570 masl, is one of the numerous extreme Andean microbial ecosystems described over recent years. Extreme ... -
BOLA-DRB3 gene polymorphisms influence bovine leukaemia virus infection levels in Holstein and Holstein × Jersey crossbreed dairy cattle
Carignano, Hugo Adrian; Beribe, Maria Jose; Caffaro, María Eugenia; Amadio, Ariel; Nani, Juan Pablo; Gutierrez, Gerónimo; Alvarez, Irene; Trono, Karina Gabriela; Miretti, Marcos Mateo; Poli, Mario Andres (2017-05)Bovine leukemia virus (BLV) infections, causing persistent lymphocytosis and lethal lymphosarcoma in cattle, have reached high endemicity on dairy farms. We observed extensive inter-individual variation in the level of ... -
Broad-range lytic bacteriophages that kill Staphylococcus aureus local field strains
Abatángelo, Virginia; Peressutti Bacci, Natalia; Boncompain, Carina A.; Amadio, Ariel; Carrasco, Soledad; Suárez, Cristian A.; Morbidoni, Héctor R. (Plos One, 2017-07)Staphylococcus aureus is a very successful opportunistic pathogen capable of causing a variety of diseases ranging from mild skin infections to life-threatening sepsis, meningitis and pneumonia. Its ability to display ... -
Búsqueda e identificación de marcadores moleculares de resistencia a mastitis bovina
Nani, Juan Pablo (Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, 2016)La mastitis es considerada la patología que produce mayores pérdidas económicas para la industria láctea en general ya que produce una disminución en la producción, afecta la calidad de la leche, la tasa de preñez, posee ... -
Characterization of novel proteases identified by metagenomic analysis from dairy stabilization ponds
Irazoqui, Jose Matias; Eberhardt, María Florencia; Santiago, Gonzalo Manuel; Amadio, Ariel (Springer, 2023-05)Cheese whey is the main by-product of dairy industries. It is used as a raw material for other value-added products, like whey protein concentrate. By using enzymes, this product can be further treated to obtain new higher ... -
Complete Sequence and Organization of pFR260, the Bacillus thuringiensis INTA Fr7-4 Plasmid Harboring Insecticidal Genes
Benintende, Graciela Beatriz; Berretta, Marcelo Facundo; Zandomeni, Ruben; Sauka, Diego Hernan; Amadio, Ariel F.; Ortiz, Elio Maximiliano; Navas, Laura Emilce (2017)We report the complete sequence and analysis of pFR260, a novel megaplasmid of 260,595 bp from the Bacillus thuringiensis strain INTA Fr7-4 isolated in Argentina. It carries 7 insecticidal genes: 3 cry8 copies previously ... -
Cost-Effective Method to Perform SARS-CoV-2 Variant Surveillance: Detection of Alpha, Gamma, Lambda, Delta, Epsilon, and Zeta in Argentina
Torres, Carolina; Mojsiejczuk, Laura; Acuña, Dolores; Alexay, Sofía; Amadio, Ariel; Aulicino, Paula; Debat, Humberto Julio; Fay, Fabián; Fernandez, Franco Daniel; Giri, Adriana A.; Goya, Stephanie; Konig, Guido Alberto; Lucero, Horacio; Nabaes Jodar, Mercedes Soledad; Pianciola, Luis; Sfalcin, Javier A.; Acevedo, Raúl M.; Bengoa Luoni, Sofía Ailin; Bolatti, Elisa M.; Brusés, Bettina; Cacciabue, Marco Polo Domingo; Casal, Pablo E.; Cerri, Agustina; Chouhy, Diego; Dus Santos, Maria Jose; Eberhardt, María Florencia; Fernandez, Ailen; Fernandez, Paula Del Carmen; Fernández Do Porto, Darío; Formichelli, Laura; Gismondi, María Ines; Irazoqui, Jose Matias; Lorenzini Campos, Melina; Lusso, Silvina; Marquez, Nathalie; Muñoz Hidalgo, Marianne Graziel; Mussin, Javier; Natale, Mónica; Oria, Griselda; Pisano, María Belén; Posner, Victoria; Puebla, Andrea Fabiana; Viegas, Mariana (Frontiers Media, 2021-12-10)The emergence of SARS-CoV-2 variants with concerning characteristics to public health has attracted the attention of the scientific community and governments both regionally and globally since the end of 2020. The most ... -
Design and evaluation of synthetic bacterial consortia for optimized phenanthrene degradation through the integration of genomics and shotgun proteomics
Macchi, Marianela; Festa, Sabrina; Nieto, Esteban; Irazoqui, Jose Matias; Vega-Vela, Nelson E.; Junca, Howard; Valacco, María Pía; Amadio, Ariel; Morelli, Irma S.; Coppotelli, Bibiana M. (Elsevier, 2021-03)Two synthetic bacterial consortia (SC) composed of bacterial strains Sphingobium sp. (AM), Klebsiella aerogenes (B), Pseudomonas sp. (Bc-h and T), Burkholderia sp. (Bk) and Inquilinus limosus (Inq) isolated from a natural ... -
Detection by multiplex PCR of Mycoplasma species associated with dairy cattle in Argentina = Detección por PCR multiplex de especies de Mycoplasma asociadas a ganado lechero en Argentina
Neder, Veronica Elizabeth; Amadio, Ariel; Calvinho, Luis Fernando (Elsevier, 2021)There is scarce information about the frequency and epidemiological and clinical features associated with the presence of Mycoplasma spp. in Argentine dairy herds. The objectives of this study were to develop a multiplex ... -
Dissecting light sensing and metabolic pathways on the millimeter scale in high-altitude modern stromatolites
Alonso-Reyes, Daniel Gonzalo; Galván, Fátima Silvina; Irazoqui, Jose Matias; Amadio, Ariel; Tschoeke, Diogo; Thompson, Fabiano; Albarracín, Virginia Helena; Farias, María Eugenia (Springer, 2022-10)Modern non-lithifying stromatolites on the shore of the volcanic lake Socompa (SST) in the Puna are affected by several extreme conditions. The present study assesses for the first time light utilization and functional ... -
Draft Genome Sequence of Bacillus thuringiensis INTA Fr7-4
Navas, Laura Emilce; Berretta, Marcelo Facundo; Ortiz, Elio Maximiliano; Sauka, Diego Hernan; Benintende, Graciela Beatriz; Zandomeni, Ruben; Amadio, Ariel (2017)We report here the complete annotated 6,035,547-bp draft genome sequence of Bacillus thuringiensis INTA Fr7-4. This strain contains three cry8 and two vip1 and vip2 insecticidal toxin genes. -
First Genome Sequence of Leptospira interrogans Serovar Pomona, Isolated from a Bovine Abortion
Varni, Vanina Delia; Koval, Ariel; Nagel, Ariel Gaston; Ruybal, Paula; Caimi, Karina Cynthia; Amadio, Ariel F. (2016)Leptospirosis is a widespread zoonosis and a re-emergent disease of global distribution with major relevance in veterinary pro- duction. Here, we report the whole-genome sequence of Leptospira interrogans serovar Pomona ... -
First report of Mycoplasma leachii isolation associated with disease in dairy calves in Argentina = Primer aislamiento de Mycoplasma leachii asociado a enfermedad en terneros de tambo en Argentina
Neder, Veronica Elizabeth; Allassia, Martín; Amadio, Ariel; Calvinho, Luis Fernando (2018)There are few reports about the isolation of Mycoplasma species associated with cattle disease in Argentina. In this work we describe the detection of Mycoplasma leachii associated with disease in dairy calves in Santa Fe ... -
Generalized glycogenosis in Brahman-derived breeds: diagnosis and prevalence in Argentina
Caffaro, María Eugenia; Raschia, Maria Agustina; Amadio, Ariel; Poli, Mario Andres (Springer, 2019-08)Generalized glycogenosis is a lethal autosomal recessive disease caused by a deficient activity of the acidic 1,4-α-glucosidase enzyme and characterized by an accumulation of glycogen within lysosomes. Three mutations in ... -
Genome-wide association study for somatic cell score in Argentinean dairy cattle
Nani, Juan Pablo; Raschia, Maria Agustina; Poli, Mario Andres; Calvinho, Luis Fernando; Amadio, Ariel (2015-05)This study aimed to understand the genomic architecture of Argentinean dairy herds by measuring linkage disequilibrium (LD) and identifying loci associated with parameters calculated from somatic cell count (SCC). Phenotypic ... -
Genome-wide scan for commons SNPs affecting bovine leukemia virus infection level in dairy cattle
Carignano, Hugo Adrian; Roldan, Dana Leticia; Beribe, Maria Jose; Raschia, Maria Agustina; Amadio, Ariel; Nani, Juan Pablo; Gutierrez, Gerónimo; Alvarez, Irene; Trono, Karina Gabriela; Poli, Mario Andres; Miretti, Marcos Mateo (2018-02-13)Background: Bovine leukemia virus (BLV) infection is omnipresent in dairy herds causing direct economic losses due to trade restrictions and lymphosarcoma-related deaths. Milk production drops and increase in the culling ... -
Genomic analysis of shiga toxin-containing Escherichia coli O157:H7 isolated from Argentinean cattle
Amadio, Ariel; Bono, James L.; Irazoqui, Jose Matias; Larzabal, Mariano; Marques Da Silva, Wanderson; Eberhardt, María Florencia; Riviere, Nahuel Agustin; Gally, David; Manning, Shannon D.; Cataldi, Angel Adrian (Plos, 2021-10)Cattle are the main reservoir of Enterohemorrhagic Escherichia coli (EHEC), with O157:H7 the distinctive serotype. EHEC is the main causative agent of a severe systemic disease, Hemolytic Uremic Syndrome (HUS). Argentina ... -
Identificación de genes codificantes de enzimas de interés industrial en una cepa de bacteria termofílica aislada de aguas termales de Salta (Argentina)
Navas, Laura Emilce; Amadio, Ariel; Fuxan Laria, Irma Noemí; Zandomeni, Ruben (Gerencia de Comunicación e Imagen Institucional, DNA SICC, INTA, 2014-04)Se aislaron dos bacterias termofílicas a partir de aguas termales de la provincia de Salta, Argentina. Estudios filogenéticos permitieron caracterizar los aislamientos como pertenecientes a los géneros Thermus y Geobacillus. ... -
Identificación de variantes alélicas de genes candidatos involucrados en producción de leche en un rodeo de bovinos de raza Holando y cruzas Holando por Jersey
Raschia, Maria Agustina (Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, 2015-07)Las razas bovinas Holando y Jersey son dos de las razas productoras de leche más importantes a nivel mundial y las principales en nuestro país. El objetivo del trabajo de tesis fue realizar un análisis exploratorio que ...