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A new cage-like particle adjuvant enhances protection of foot-and-mouth disease vaccine
Bidart, Juan Esteban; Kornuta, Claudia Alejandra; Gammella, Mariela; Gnazzo, Victoria; Soria, Ivana; Langellotti, Cecilia Ana; Mongini, Claudia; Galarza, Roxana Ivon; Calvinho, Luis Fernando; Lupi, Giuliana; Quattrocchi, Valeria; Marcipar, Ivan; Zamorano, Patricia Ines (Frontiers Media, 2020-07)Foot-and-Mouth Disease (FMD) is an acute viral disease that causes important economy losses. Vaccines with new low-cost adjuvants that stimulate protective immune responses are needed and can be assayed in a mouse model ... -
A safe and effective vaccine against bovine leukemia virus
Suarez Archilla, Guillermo; Gutierrez, Gerónimo; Camussone, Cecilia; Calvinho, Luis Fernando; Abdala, Alejandro Ariel; Alvarez, Irene; Petersen, Marcos; Franco, Lautaro Nahuel; De Stefano, Gabriel Alejandro; Monti, Gustavo; Jacques, Jean-Rock; Joris, Thomas; Willems, Luc; Trono, Karina Gabriela (Frontiers Media, 2022-08)Previous attempts to develop a vaccine against bovine leukemia virus (BLV) have not been successful because of inadequate or short-lived stimulation of all immunity components. In this study, we designed an approach based ... -
Aislamiento y caracterización de Corynebacterium pseudotuberculosis biotipo ovis en Lama glama en Córdoba, Argentina.
Auad, J.; Cooper, Laura G.; Cerutti, Julieta; Marcellino, Romanela; Neder, Veronica Elizabeth; Calvinho, Luis Fernando (2017)Corynebacterium pseudotuberculosis es el agente causal de la linfadenitis caseosa en distintas especies animales. No existen reportes en Argentina de casos clínicos en camélidos. Esta comunicación describe el aislamiento ... -
Antimicrobial resistance of Staphylococcus aureus isolated from bovine mastitis: Systematic review and meta-analysis
Molineri, Ana Ines; Camussone, Cecilia; Zbrun, María Virginia; Suarez Archilla, Guillermo; Cristiani, Mariana; Neder, Veronica Elizabeth; Calvinho, Luis Fernando; Signorini Porchiett, Marcelo Lisandro (Elsevier, 2021-03)Staphylococcus aureus, one of the main contagious mastitis pathogens worldwide, is characterized for causing chronic intramammary infections that respond poorly to antimicrobial therapy, disseminating within the herd leading ... -
Antimicrobial resistance of Streptococcus uberis isolated from bovine mastitis: Systematic review and meta-analysis
Miotti, Camila; Cicotello, Joaquin; Suarez Archilla, Guillermo; Neder, Veronica Elizabeth; Alvarado, Wanda; Calvinho, Luis Fernando; Signorini Porchiett, Marcelo Lisandro; Camussone, Cecilia; Zbrun, María Virginia; Molineri, Ana Ines (Elsevier, 2023-11)Streptococcus uberis is one of the most common pathogens associated with bovine mastitis, commonly treated with antimicrobials (AM), favoring the appearance of antimicrobial resistance (AMR). The objective of this work was ... -
Antimicrobial resistance trends of Staphylococcus aureus isolated from bovine intramammary infections from 1990 to the present time in Argentina
Molineri, Ana Ines; Neder, Veronica Elizabeth; Calvinho, Luis Fernando (2018)Staphylococcus aureus is one of the most prevalent major mastitis pathogens in dairy herds worldwide. Antimicrobial therapy is one of the bases of S. aureus mastitis control programmes, both for subclinical cases at drying ... -
Aspectos de la respuesta inmune innata en las infecciones intramamarias causadas por Staphylococcus aureus en bovinos = Aspects of the innate immune response to intramammary Staphylococcus aureus infections in cattle
Pereyra, Elizabet Amanda Lorena; Dallard, Bibiana Elisabet; Calvinho, Luis Fernando (2014-10)Staphylococcus aureus es el principal agente causante de mastitis bovina en Argentina y en el mundo. Esta bacteria ocasiona infecciones crónicas que generan importantes pérdidas a los productores y la industria lechera. ... -
Assessment of the potential utility of different regions of Streptococcus uberis adhesion molecule (SUAM) for mastitis subunit vaccine development
Perrig, Melina Soledad; Veaute, Carolina Melania Isabel; Renna, María Sol; Pujato, Nazarena; Calvinho, Luis Fernando; Marcipar, Ivan Sergio; Barbagelata, Maria Sol (2017-04)Streptococcus uberis is one of the most prevalent pathogens causing clinical and subclinical mastitis worldwide. Among bacterial factors involved in intramammary infections caused by this organism, S. uberis adhesion ... -
BLV: lessons on vaccine development
Abdala, Alejandro Ariel; Alvarez, Irene; Brossel, Hélène; Calvinho, Luis Fernando; Carignano, Hugo; Franco, Lautaro Nahuel; Gazon, Hélène; Gillissen, Christelle; Hamaidia, Malik; Hoyos, Clotilde; Jacques, Jean-Rock; Joris, Thomas; Laval, Florent; Petersen, Marcos Iván; Porquet, Florent; Porta, Natalia Gabriela; Ruiz, Vanesa; Safari, Rogaiyeh; Suarez Archilla, Guillermo Alejandro; Trono, Karina Gabriela; Willems, Luc (BMC, 2019-10-07)Vaccination against retroviruses is a challenge because of their ability to stably integrate into the host genome, undergo long-term latency in a proportion of infected cells and thereby escape immune response. Since ... -
Brote de mastitis clínica por Corynebacterium spp. y Streptococcus dysgalactiae en cabras en Salta, Argentina
Micheloud, Juan Francisco; Neder, Veronica Elizabeth; Nuovo, F.; Suarez, Victor Humberto; Calvinho, Luis Fernando (Gerencia de Comunicación e Imagen Institucional, DNA SICC, INTA, 2014-04)Las infecciones mamarias son un problema grave para la producción lechera en cabras a nivel mundial. Staphylococcus spp. es el patógeno más prevalente en las infecciones intramamarias de los pequeños rumiantes, sin embargo, ... -
Búsqueda e identificación de marcadores moleculares de resistencia a mastitis bovina
Nani, Juan Pablo (Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, 2016)La mastitis es considerada la patología que produce mayores pérdidas económicas para la industria láctea en general ya que produce una disminución en la producción, afecta la calidad de la leche, la tasa de preñez, posee ... -
Capacity of two Staphylococcus aureus strains with different adaptation genotypes to persist and induce damage in bovine mammary epithelial cells and to activate macrophages
Sacco, Sofía Clara; Velázquez, Natalia S.; Renna, María Sol; Beccaria, Camila; Baravalle, Celina; Pereyra, Elizabet Amanda Lorena; Monecke, Stefan; Calvinho, Luis Fernando; Dallard, Bibiana Elisabet (Elsevier, 2020-01)The aim of this study was to evaluate and compare the ability to adhere/internalize, persist, and induce damage in mammary epithelial cells (MAC-T) of two Staphylococcus aureus strains with different adaptation genotypes ... -
Características de la leche y del queso durante la lactancia de cabras sin infecciones intramamarias
Suarez, Victor Humberto; Martinez, Gabriela Marcela; Fili, Josefina Marcela; Gianre, Vilma Rita; Calvinho, Luis Fernando; Alfaro, Rodolfo Jose (2014-11)El objetivo de este estudio fue analizar a lo largo de la lactancia, las relaciones entre el conteo de células somáticas (CCS) y los componentes de la leche con respecto a factores fisiológicos y a la calidad de la leche ... -
Characterization of immune response in Staphylococcus aureus chronically infected bovine mammary glands during active involution
Andreotti, Carolina Soledad; Baravalle, Celina; Sacco, Sofía Clara; Lovato, Melisa Belen; Pereyra, Elizabet Amanda Lorena; Renna, María Sol; Ortega, Hugo Hector; Calvinho, Luis Fernando; Dallard, Bibiana Elisabet (2017-10)The aim of this study was to characterize the immune response in Staphylococcus aureus chronically infected bovine mammary glands during active involution. Twenty-one Holstein non-pregnant cows in late lactation either ... -
Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections
Russi, Norma B.; Maito, Julia; Dieser, Silvana Andrea; Renna, Maria Sol; Signorini, Marcelo; Camussone, Cecilia; Neder, Veronica Elizabeth; Pol, Martín; Tirante, Liliana; Odierno, Liliana Mónica; Calvinho, Luis Fernando (2015-08)Few studies have described the relationship between genotypic and phenotypic methods for detecting penicillin resistance in Staphylococcus aureus isolated from bovine intramammary infection (IMI). Six phenotypic methods ... -
Desarrollo de un inmunógeno prototípico para el control de mastitis bovinas causadas por Staphylococcus aureus
Camussone, Cecilia (Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 2012)Staphylococcus aureus es el patógeno más frecuentemente aislado de casos de mastitis bovina en Argentina y el mundo. Las particulares características de las infecciones intramamarias por S. aureus, han llevado a la búsqueda ... -
Detection by multiplex PCR of Mycoplasma species associated with dairy cattle in Argentina = Detección por PCR multiplex de especies de Mycoplasma asociadas a ganado lechero en Argentina
Neder, Veronica Elizabeth; Amadio, Ariel; Calvinho, Luis Fernando (Elsevier, 2021)There is scarce information about the frequency and epidemiological and clinical features associated with the presence of Mycoplasma spp. in Argentine dairy herds. The objectives of this study were to develop a multiplex ... -
Detection of Staphylococcus aureus adhesion and biofilm-producing genes and their expression during internalization in bovine mammary epithelial cells
Pereyra, Elizabet Amanda Lorena; Renna, María Sol; Picech, Florencia; Baravalle, Celina; Andreotti, Carolina Soledad; Russi, Romina; Calvinho, Luis Fernando; Diez, Cristina; Dallard, Bibiana Elisabet (2016)Staphylococcus aureus is one of the most prevalent pathogens isolated from bovine mastitis, causing chronic intramammary infections (IMI) that limit profitable dairying. The course of infection is often associated with ... -
Development and assessment of a new cage‐like particle adjuvant
Bertona, Daiana; Pujato, Nazarena; Bontempi, Iván; González, Verónica Doris Guadalupe; Cabrera, Gabriel Gustavo; Gugliotta, Luis Marcelino; Hozbor, Daniela Flavia; Nicastro, Alcides; Calvinho, Luis Fernando; Marcipar, Iván Sergio (2017-10)Objectives: To obtain and assess stable cage‐like particles with low surface charge density, which can be prepared using a standardized, economic and scalable method. Methods: To form these nanoparticles, the lipid ... -
Differential immune response to two Staphylococcus aureus strains with distinct adaptation genotypes after experimental intramammary infection of dairy cows
Engler, Carolina; Renna, María Sol; Beccaria, Camila; Silvestrini, Paula; Pirola, Silvana I.; Pereyra, Elizabet Amanda Lorena; Baravalle, Celina; Camussone, Cecilia; Monecke, Stefan; Calvinho, Luis Fernando; Dallard, Bibiana Elisabet (Elsevier, 2022-11)The aim of this study was to evaluate and compare the ability of two S. aureus strains with different adaptation genotypes (low and high) to the bovine mammary gland (MG) to establish an intramammary infection (IMI) and ...